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Related Concept Videos

Coagulation01:06

Coagulation

280
Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
280

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Related Experiment Video

Updated: Jun 20, 2025

Author Spotlight: Advancing Research in Microbial Autoaggregation Using Imaging Flow Cytometry
05:19

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Elucidating bacterial coaggregation through a physicochemical and imaging surface characterization.

Ana C Afonso1, Jack Botting2, Inês B Gomes3

  • 1LEPABE - Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr Roberto Frias, 4200-465 Porto, Portugal; ALiCE - Associate Laboratory in Chemical Engineering, Faculty of Engineering, University of Porto, Rua Dr Roberto Frias, 4200-465 Porto, Portugal; CITAB, Department of Veterinary Sciences, University of Trás-os-Montes e Alto Douro, 5000-801 Vila Real, Portugal; CEB-LABBELS, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.

The Science of the Total Environment
|July 20, 2024
PubMed
Summary
This summary is machine-generated.

Bacterial coaggregation in aquatic systems is driven by pili-like adhesins on Delftia acidovorans 005P. These unique structures, along with surface properties, mediate cell-cell interactions crucial for biofilm formation and water quality.

Keywords:
Cell-cell interactionCo-adhesion energyCryo-electron tomographyDelftia acidovoransFTIRXDLVO theory

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Area of Science:

  • Microbiology
  • Surface Chemistry
  • Biophysics

Background:

  • Bacterial coaggregation, a specific cell-cell interaction, is well-understood in oral bacteria but poorly characterized in aquatic environments.
  • Understanding coaggregation mechanisms is vital due to its impact on biofilms, water quality, engineered systems, and biotechnology.
  • This study investigates the factors contributing to coaggregation in Delftia acidovorans, a drinking water isolate.

Purpose of the Study:

  • To comprehensively characterize the cell surface of a coaggregating strain (Delftia acidovorans 005P) and a non-coaggregating strain (D. acidovorans 009P).
  • To identify the specific surface factors responsible for the coaggregation ability of strain 005P.

Main Methods:

  • Physicochemical characterization of bacterial surface properties.
  • Fourier-transform infrared spectroscopy (FTIR) for chemical analysis.
  • Transmission electron microscopy (TEM) and cryo-electron tomography (cryo-ET) for high-resolution imaging of cell structures.

Main Results:

  • The coaggregating strain (005P) showed higher surface hydrophobicity, negative surface charge, and co-adhesion energies compared to the non-coaggregating strain (009P).
  • FTIR revealed subtle differences in carbohydrates and proteins (phosphodiesters/amide III) between the strains.
  • Cryo-ET identified significant variations in pili structures, with 005P possessing more frequent and distinct pili-like adhesins.

Conclusions:

  • The coaggregation ability of Delftia acidovorans 005P is directly linked to its unique pili-like adhesins.
  • These pili act as key mediators of cell-cell interactions in aquatic environments.
  • This research provides critical insights into microbial coaggregation mechanisms in water systems.