Related Experiment Video
Updated: Jun 20, 2025
07:22
Minimally Invasive Murine Laryngoscopy for Close-Up Imaging of Laryngeal Motion During Breathing and Swallowing
Published on: December 1, 2023
475
Precision-Targeted Injection Laryngoplasty and Longitudinal Biomaterial Effects Evaluation Using High-resolution
Wen-Hsuan Tseng1,2, Tzu-Yu Hsiao1, Tsung-Lin Yang1,2,3,4
1Department of Otolaryngology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
Summary
A novel ultrasound-guided surgical model for laryngeal injection in rats was developed. This method allows for precise injection and monitoring of vocal fold medialization, aiding in the development of new treatments for glottic insufficiency.
Area of Science:
- Otolaryngology
- Regenerative Medicine
- Medical Imaging
Background:
- Current transoral laryngeal injection models have limitations including poor visualization and restricted workspace.
- These limitations hinder the investigation of injectable materials for laryngeal interventions.
- There is a need for improved animal models for evaluating laryngeal therapies.
Purpose of the Study:
- To develop and validate a novel ultrasound-guided surgical model for laryngeal injection in rats.
- To establish a systemic evaluation approach for laryngeal intervention therapy.
- To assess the feasibility of using this model for screening therapeutic agents.
Main Methods:
- Injection laryngoplasty with hyaluronic acid was performed on rats with induced unilateral vocal fold paralysis.
- Ultra-high frequency ultrasound imaging guided the injections and enabled longitudinal follow-up.
- Measurements included glottic area, glottic angle, and vocal fold width ratio, supplemented by laryngoscopy and histology.
Main Results:
- Successful unilateral injections into the paralyzed vocal fold were confirmed via ultrasound, laryngoscopy, and histology.
- A significant increase in vocal fold width ratio was observed for 4 weeks post-injection.
- The glottic airway area remained unchanged, indicating targeted augmentation.
Conclusions:
- A novel, ultrasound-guided rat model for laryngeal injection was successfully established.
- This model provides precise injection guidance and robust documentation of therapeutic effects.
- The methodology is suitable for screening novel therapeutic agents for glottic insufficiency.
