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Updated: Jul 10, 2026

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Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry
Published on: March 18, 2012
[Hydrogenase activity in Brevibacterium flavum]
Mikrobiologiia
|July 1, 1985
Abstract:
The activity of hydrogenase was assayed in the intact cells and subcellular fractions of Brevibacterium flavum. The organism was shown to have the membrane-bound form of hydrogenase. The soluble NAD+-reducing hydrogenase was not found. Oxygen inhibited the hydrogenase activity, and its action was reversible. Molecular hydrogen activated the hydrogenase of B. flavum, which was shown to be a constitutive enzyme.
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