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Automated modification of the Ames test with COBAS Bact
Mutation Research
|November 1, 1985
Summary
Automating the Salmonella mutagenicity test with the COBAS Bact system and a Hewlett-Packard computer showed good agreement with the standard Ames test for over 30 compounds.
Area of Science:
- Microbiology
- Toxicology
- Genetics
Background:
- The Salmonella mutagenicity test (Ames test) is a standard method for assessing the mutagenic potential of chemical compounds.
- Automation of laboratory procedures can improve efficiency and reproducibility.
Purpose of the Study:
- To automate the Salmonella mutagenicity test using the COBAS Bact system.
- To evaluate the performance of the automated system compared to the standard Ames test.
Main Methods:
- The COBAS Bact automatic analyser for microbiology was utilized.
- A Hewlett-Packard 9816S computer system supplemented the analyser.
- Over 30 different chemical compounds were tested using the automated system.
Main Results:
- The automated system successfully performed the Salmonella mutagenicity test.
- Results from the automated system demonstrated good agreement with the standard Ames test.
- The system proved effective for testing a diverse range of compounds.
Conclusions:
- The automated COBAS Bact system is a viable and effective tool for conducting the Salmonella mutagenicity test.
- Automation provides reliable results comparable to the traditional Ames test, enhancing throughput and consistency in mutagenicity testing.