Related Experiment Video
Updated: Jun 20, 2025
Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
Published on: January 2, 2013
A method for selecting P antigen-negative red cell units for patients with paroxysmal cold hemoglobinuria
Geethika Sajeewani Manchanayake1
1Blood Bank, District General Hospital, Monaragala, Sri Lanka.
In the absence of specific antisera and molecular typing technique, selection of P antigen-negative red cell unit is a challenge. This article explains a new technique that can be used in emergency situations to screen P antigen-negative red cell unit for the transfusion of patients with paroxysmal cold hemoglobinuria tested positive for Donath-Landsteiner antibody. The technique is based on the theory behind the Donath-Landsteiner test, and it can be performed even in a transfusion laboratory with minimum facilities.
In the absence of specific antisera and molecular typing technique, selection of P antigen-negative red cell unit is a challenge. This article explains a new technique that can be used in emergency situations to screen P antigen-negative red cell unit for the transfusion of patients with paroxysmal cold hemoglobinuria tested positive for Donath-Landsteiner antibody. The technique is based on the theory behind the Donath-Landsteiner test, and it can be performed even in a transfusion laboratory with minimum facilities.
