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Surgical Lumbar Sympathectomy in Mice
1Medical Scientist Training Program, Emory University School of Medicine; Neuroscience Graduate Program, Laney Graduate School, Emory University; Department of Cell Biology, Emory University School of Medicine.
Journal of Visualized Experiments : Jove
|July 22, 2024
Summary
This study introduces a surgical method to remove lumbar sympathetic ganglia in mice, effectively reducing sympathetic nerve function in the hind limbs. This technique aids research into peripheral nerve injury and sympathetic nervous system roles.
Area of Science:
- Neuroscience
- Surgical techniques
- Peripheral nervous system research
Background:
- Peripheral nerve injuries are prevalent, with limited functional recovery in most patients.
- The role of the sympathetic nervous system in peripheral nerve injury is understudied.
- Current methods for studying sympathetic innervation have limitations.
Purpose of the Study:
- To develop a reliable surgical model for investigating sympathetic innervation in peripheral targets.
- To specifically target and assess the function of postganglionic sympathetic neurons innervating the hind limbs.
- To provide a viable alternative to chemical sympathectomy for studying sympathetic nervous system roles.
Main Methods:
- Surgical removal of L2-L5 lumbar sympathetic ganglia in mice.
- Development of a survival surgery technique for small animals.
- Assessment of hind paw sweat response and sciatic nerve sympathetic axon density post-surgery.
Main Results:
- The surgical lumbar sympathectomy reliably decreased hind paw sweat response.
- A significant reduction in sympathetic axons within the sciatic nerve was observed.
- The surgical model proved effective in targeting postganglionic sympathetic neurons.
Conclusions:
- Surgical lumbar sympathectomy in mice is a practicable and specific method.
- This model allows for detailed study of sympathetic innervation in peripheral nerve injury.
- The technique offers a valuable tool for exploring sympathetic nervous system functions in the periphery.
