Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

¹H NMR: Interpreting Distorted and Overlapping Signals01:02

¹H NMR: Interpreting Distorted and Overlapping Signals

1.0K
Spin systems where the difference in chemical shifts of the coupled nuclei is greater than ten times J are called first-order spin systems. These nuclei are weakly coupled, and their chemical shifts and coupling constant can generally be estimated from the well-separated signals in the spectrum.
As Δν decreases and the signals move closer, the doublets appear increasingly distorted. The intensities of the inner lines increase at the cost of those of the outer lines as the signals are...
1.0K
¹H NMR of Conformationally Flexible Molecules: Temporal Resolution00:52

¹H NMR of Conformationally Flexible Molecules: Temporal Resolution

824
At room temperature, the chair conformer of cyclohexane undergoes rapid ring flipping between two equivalent chair conformers at a rate of approximately 105 times per second. These two chair conformers are in equilibrium. The rapid ring flipping results in the interconversion of the axial proton to an equatorial proton and an equatorial to the axial proton. Such interconversions are too rapid and cannot be detected on the NMR timescale. Hence, the NMR spectrometer cannot distinguish between the...
824
2D NMR: Homonuclear Correlation Spectroscopy (COSY)01:06

2D NMR: Homonuclear Correlation Spectroscopy (COSY)

1.0K
Homonuclear correlation spectroscopy, or COSY, is a 2-dimensional NMR technique that provides information about coupled protons. Typically, the geminal and vicinal coupling are observed. For example, consider the COSY spectrum of ethyl acetate, where its 1D proton NMR spectrum is plotted along the vertical and horizontal axes with their corresponding chemical shift scale. Three spots on the diagonal corresponding to the three peaks in the 1D proton spectrum are called diagonal peaks. The COSY...
1.0K
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

1.1K
The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
1.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Toward Hydrogen Isotope Separations through Strong Hydrogen Adsorption at Open Copper(I) Sites in an Ultramicroporous Metal-Organic Framework.

Journal of the American Chemical Society·2026
Same author

Microhydration and Interfacial Activity of Triarylmethane Dyes at the Air-Water Interface.

The journal of physical chemistry. B·2026
Same author

Electrolyte-Dependent, "Microscopically Irreversible" H-Atom Transfer Kinetics of Ce-Based Metal-Organic Framework, Ce-MOF-808.

ACS applied materials & interfaces·2026
Same author

New Insights Into Curcumin Behavior After Its Biosynthesis in Engineered Escherichia coli Through Spectroscopic and Spatial Analysis.

Phytochemical analysis : PCA·2025
Same author

Beyond real: alternative unitary cluster Jastrow models for molecular electronic structure calculations on near-term quantum computers.

Chemical science·2025
Same author

Synthesis of an Oxo-Bridged Fe(III) Porphyrin(2.1.2.1) Dimer and Its Enhanced Electrocatalytic Oxygen Evolution.

Inorganic chemistry·2025

Related Experiment Video

Updated: Jun 20, 2025

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
10:35

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Published on: May 29, 2018

8.7K

Conical Intersections at Interfaces Revealed by Phase-Cycling Interface-Specific Two-Dimensional Electronic

Zhi-Chao Huang-Fu1, Nikolay V Tkachenko1, Yuqin Qian1

  • 1Department of Chemistry and Biochemistry, Utah State University, Logan, Utah 84322, United States.

Journal of the American Chemical Society
|July 22, 2024
PubMed
Summary

Researchers developed a new spectroscopy technique to study conical intersections (CIs) at interfaces. This method reveals how molecular orientation at interfaces alters photochemical reaction pathways compared to bulk water.

More Related Videos

Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid
08:54

Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid

Published on: January 25, 2020

5.6K
Author Spotlight: Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems
06:54

Author Spotlight: Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems

Published on: June 23, 2023

823

Related Experiment Videos

Last Updated: Jun 20, 2025

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals
10:35

Novel Techniques for Observing Structural Dynamics of Photoresponsive Liquid Crystals

Published on: May 29, 2018

8.7K
Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid
08:54

Vibrational Spectra of a N719-Chromophore/Titania Interface from Empirical-Potential Molecular-Dynamics Simulation, Solvated by a Room Temperature Ionic Liquid

Published on: January 25, 2020

5.6K
Author Spotlight: Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems
06:54

Author Spotlight: Advances in Nanoscale Infrared Spectroscopy to Explore Multiphase Polymeric Systems

Published on: June 23, 2023

823

Area of Science:

  • Photochemistry
  • Surface Science
  • Molecular Dynamics

Background:

  • Conical intersections (CIs) are crucial for controlling molecular photochemical reactions.
  • Characterizing CIs at interfaces and surfaces is currently challenging.
  • Understanding interfacial molecular dynamics is vital for chemical processes.

Purpose of the Study:

  • To develop a novel tool for characterizing CIs at the air/water interface.
  • To investigate the nonadiabatic dynamics of molecules at interfaces using advanced spectroscopy and modeling.
  • To compare interfacial and bulk photochemical reaction pathways.

Main Methods:

  • Developed phase-cycling interface-specific two-dimensional electronic spectroscopy (i2D-ES).
  • Integrated phase-locked pump pulse pairs with an interface-specific electronic probe.
  • Employed advanced computational modeling to explore nonadiabatic dynamics.
  • Studied an interface-active azo dye molecule at the air/water interface.

Main Results:

  • Demonstrated distinct kinetic pathways for nonadiabatic transitions at the interface versus bulk water.
  • Identified two conical intersections: S2-S1 and S1-S0.
  • Found differences in ground-state molecular conformations at the interface.
  • Observed slower nonadiabatic dynamics at the interface due to excited-state configurations.
  • Noted significantly longer S1 dark state to S0 ground state transitions at the interface.

Conclusions:

  • Molecular orientation at interfaces significantly influences photochemical reaction pathways.
  • The developed i2D-ES technique is effective for characterizing CIs at interfaces.
  • Interfacial environments lead to altered nonadiabatic dynamics compared to bulk phases.
  • Understanding these interfacial effects is key for controlling chemical reactions on surfaces.