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Complete genome sequence of Microbacterium foliorum phage Curie, a podovirus isolated from soil in Spokane,

Emma N Horton1, Erika K Beach1, Kathryn T Cook1

  • 1Department of Biology, Gonzaga University , Spokane, Washington, USA.

Microbiology Resource Announcements
|July 22, 2024
PubMed

Insights

Bacteriophage Curie, a novel podovirus, infects Microbacterium foliorum. Its genome analysis reveals similarities to phi29-like phages, establishing a new cluster with phage PineapplePizza.

Area of Science:

  • Microbiology
  • Virology
  • Genomics

Background:

  • Bacteriophages are viruses that infect bacteria.
  • Actinobacteriophages are bacteriophages that infect Actinobacteria.
  • Phylogenetic analysis is crucial for classifying novel phages.

Purpose of the Study:

  • To characterize the genome of Bacteriophage Curie.
  • To determine the phylogenetic relationship of Bacteriophage Curie to other known phages.
  • To establish a new phage cluster based on genomic data.

Main Methods:

  • Genome sequencing of Bacteriophage Curie.
  • Bioinformatic analysis of the Curie genome.
  • Comparative genomic analysis with related phages.

Main Results:

  • The Curie genome is 16,810 bp with 90 bp terminal inverted repeats and 23 protein-coding genes.
  • Curie shares genome architecture similarities with phi29-like phages, including phage PineapplePizza.
  • Bacteriophage Curie and PineapplePizza form a new actinobacteriophage cluster, designated Cluster GI.

Conclusions:

  • Bacteriophage Curie represents a distinct lineage within actinobacteriophages.
  • Genomic data supports the classification of Curie and PineapplePizza into a new cluster (GI).
  • This study expands the known diversity of actinobacteriophages.
Keywords:
bacteriophages

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