Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Packaging00:58

DNA Packaging

Overview
DNA Packaging00:58

DNA Packaging

Overview
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
Nucleosome Remodeling02:54

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...
DNA Topoisomerases02:02

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types.  Type I...
DNA as a Genetic Template02:05

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Perceptual ocular dominance plasticity is resilient to concurrent attentional demands in working memory.

Vision research·2026
Same author

Transient evolution and established states of color domains in fiber lasers with a doublet radiation spectrum.

Physical review. E·2026
Same author

Earthworm-mediated partitioning of PFOA and PFOS among solid, dissolved organic, and biological phases during sludge vermicomposting.

Bioresource technology·2026
Same author

Perturbation Solutions for Laminar Flow of a Carreau-Yasuda Fluid in Annular Pipes.

Annals of the New York Academy of Sciences·2026
Same author

Deep-learning-based radiomics of intratumoral and peritumoral CT images to differentiate benign from malignant parotid tumors.

American journal of cancer research·2026
Same author

Rational design of topology-defined DNA nanoframeworks for antigen delivery and cross-presentation.

Journal of nanobiotechnology·2026

Related Experiment Video

Updated: May 8, 2026

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

14.5K

Modulated Cell Internalization Behavior of Icosahedral DNA Framework with Programmable Surface Modification.

Kui Huang1, Qiulan Yang1, Min Bao1

  • 1Institute of Molecular Medicine and Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, State Key Laboratory of Oncogenes and Related Genes, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China.

Journal of the American Chemical Society
|July 22, 2024
PubMed
Summary
This summary is machine-generated.

Surface modifications on DNA nanostructures significantly impact their cellular uptake. Understanding ligand type, valence, and pattern is key for effective targeted gene and drug delivery using these nanovehicles.

More Related Videos

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
11:05

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

7.5K
Simple, Affordable, and Modular Patterning of Cells using DNA
08:59

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

4.1K

Related Experiment Videos

Last Updated: May 8, 2026

Folding and Characterization of a Bio-responsive Robot from DNA Origami
07:59

Folding and Characterization of a Bio-responsive Robot from DNA Origami

Published on: December 3, 2015

14.5K
Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography
11:05

Functional Surface-immobilization of Genes Using Multistep Strand Displacement Lithography

Published on: October 25, 2018

7.5K
Simple, Affordable, and Modular Patterning of Cells using DNA
08:59

Simple, Affordable, and Modular Patterning of Cells using DNA

Published on: February 24, 2021

4.1K

Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Cell Biology

Background:

  • Surface modification of nanovehicles can improve cellular internalization for targeted delivery.
  • Parameters like ligand recognition, valence, and pattern influence nanostructure endocytosis, particularly for DNA nanostructures.

Purpose of the Study:

  • To systematically investigate how surface modification parameters affect the cell internalization efficiency of DNA nanostructures.
  • To elucidate the endocytic pathways and post-internalization fate of modified DNA nanostructures.

Main Methods:

  • Designed and programmed an icosahedral DNA framework with three distinct ligand types.
  • Varied ligand valence and spatial distribution on the nanostructure surface.
  • Analyzed cell internalization efficiency, endocytic pathways, and intracellular fate.

Main Results:

  • Demonstrated that ligand type, valence, and spatial arrangement significantly influence nanostructure internalization.
  • Identified specific endocytic pathways associated with different surface modifications.
  • Provided insights into the post-internalization behavior of DNA nanostructures.

Conclusions:

  • The study deepens the understanding of the relationship between surface modification and cell entry mechanisms for DNA nanostructures.
  • Offers crucial insights for designing optimized DNA framework nanostructures for targeted cell delivery applications.