Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Protein Modifications in the RER01:26

Protein Modifications in the RER

5.1K
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
5.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Zika virus recruits karyopherin α6 for efficient replication via NS2B.

Journal of virology·2026
Same author

COVID-19-associated neuroinflammation and astrocyte death in the brain linked to ORF3a-induced activation of Sur1-mediated ion channels.

mBio·2025
Same author

RNA Helicase DDX3 Interacts with the Capsid Protein of Hepatitis E Virus and Plays a Vital Role in the Viral Replication.

Pathogens (Basel, Switzerland)·2025
Same author

SARS-CoV-2 ORF3a induces COVID-19-associated kidney injury through HMGB1-mediated cytokine production.

mBio·2024
Same author

Langat virus inhibits the gp130/JAK/STAT signaling by reducing the gp130 protein level.

Journal of medical virology·2024
Same author

A novel diG motif in ORF3a protein of SARS-Cov-2 for intracellular transport.

Frontiers in cell and developmental biology·2022

Related Experiment Video

Updated: Jun 20, 2025

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
10:28

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks

Published on: June 26, 2020

9.7K

Oxysterol binding protein (OSBP) contributes to hepatitis E virus replication.

Shaoli Lin1, Peixi Chang1, Shane Tsao2

  • 1Molecular Virology Laboratory, VA-MD College of Veterinary Medicine, University of Maryland, College Park, MD, USA.

Virology Journal
|July 22, 2024
PubMed
Summary

Hepatitis E virus (HEV) replication is reduced when Oxysterol Binding Protein (OSBP) is silenced. HEV helicase interacts with OSBP, impacting its cellular localization and aiding viral RNA synthesis.

Keywords:
HelicaseHepatitis E virus (HEV)Oxysterol binding protein (OSBP)Viral replication

More Related Videos

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
11:34

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

Published on: May 10, 2022

2.2K
A Protocol for Analyzing Hepatitis C Virus Replication
13:04

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

24.1K

Related Experiment Videos

Last Updated: Jun 20, 2025

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks
10:28

A Cell Culture Model for Producing High Titer Hepatitis E Virus Stocks

Published on: June 26, 2020

9.7K
A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target
11:34

A Competent Hepatocyte Model Examining Hepatitis B Virus Entry through Sodium Taurocholate Cotransporting Polypeptide as a Therapeutic Target

Published on: May 10, 2022

2.2K
A Protocol for Analyzing Hepatitis C Virus Replication
13:04

A Protocol for Analyzing Hepatitis C Virus Replication

Published on: June 26, 2014

24.1K

Area of Science:

  • Virology
  • Molecular Biology
  • Cell Biology

Background:

  • Hepatitis E virus (HEV) causes acute infections and encodes a polyprotein with helicase activity essential for viral RNA synthesis.
  • Oxysterol Binding Protein (OSBP) regulates lipid transfer between cellular organelles, impacting cholesterol homeostasis.
  • The interaction between HEV proteins and host cellular factors is not fully understood.

Purpose of the Study:

  • To investigate the role of Oxysterol Binding Protein (OSBP) in Hepatitis E virus (HEV) replication.
  • To elucidate the interaction between the HEV helicase and OSBP.
  • To understand how OSBP influences HEV-host cell interactions.

Main Methods:

  • RNA interference (RNAi) was used to silence OSBP expression.
  • HEV replication levels were quantified following OSBP knockdown.
  • Co-immunoprecipitation and co-localization assays were performed to study protein interactions.
  • Cellular localization of OSBP was monitored in the presence of HEV helicase.

Main Results:

  • RNAi-mediated silencing of OSBP significantly reduced HEV replication.
  • The HEV helicase was found to interact with OSBP.
  • Co-localization studies confirmed the interaction between HEV helicase and OSBP in cells.
  • HEV helicase inhibited the preferential translocation of OSBP to the Golgi apparatus.

Conclusions:

  • OSBP plays a crucial role in supporting HEV replication.
  • The HEV helicase interacts with OSBP and modulates its cellular localization.
  • These findings enhance the understanding of HEV-host cell interactions and viral replication mechanisms.