JoVE - Journal of Visualized Experiments
JoVE Visualize
Contact Us

OxLDL as a prognostic biomarker of plaque instability in patients qualified for carotid endarterectomy

  • 0Department of Internal Diseases and Clinical Pharmacology, Laboratory of Tissue Immunopharmacology, Medical University of Lodz, Lodz, Poland.
Journal of Cellular and Molecular Medicine +

|

July 23, 2024

|

July 23, 2024

View abstract on PubMed

Summary

This summary is machine-generated.

Oxidized LDL cholesterol (ox-LDL) and other biomarkers can predict atherosclerotic plaque instability, a key stroke risk factor. Elevated serum ox-LDL levels are a strong independent predictor of unstable plaques, aiding in timely intervention.

Area Of Science

  • Cardiovascular Research
  • Biomarker Discovery
  • Stroke Prevention

Background

  • Atherosclerotic plaque instability is a major risk factor for stroke.
  • Early identification of unstable plaques can expedite carotid endarterectomy (CEA) and reduce vascular events.
  • Prognostic markers are needed to assess plaque vulnerability.

Purpose Of The Study

  • To evaluate the diagnostic value of serum oxidized LDL cholesterol (ox-LDL), matrix metalloproteinase 9 (MMP-9), and 8-hydroxy-2'-deoxyguanosine (8-OHdG) as prognostic markers of atherosclerotic plaque instability.
  • To determine the predictive accuracy of these biomarkers in patients undergoing CEA.

Main Methods

  • Serum samples were collected from 67 patients undergoing CEA.
  • Levels of ox-LDL, MMP-9, and 8-OHdG were measured using ELISA.
  • Receiver Operating Characteristic (ROC) curve analysis was employed to determine predictive values and optimal cut-off points.

Main Results

  • Patients with unstable plaques exhibited significantly higher serum levels of ox-LDL, MMP-9, and 8-OHdG compared to those with stable plaques.
  • Optimal cut-off points were identified: ox-LDL >31.14 ng/mL (AUC 0.86, 91.18% sensitivity, 78.79% specificity), MMP-9 >113.1 ng/mL (78.6% probability), and 8-OHdG >2.15 ng/mL (64.7% probability).
  • Multivariate regression analysis confirmed ox-LDL as an independent predictor of plaque instability.

Conclusions

  • Serum ox-LDL, MMP-9, and 8-OHdG levels are elevated in patients with unstable atherosclerotic plaques.
  • Oxidized LDL cholesterol demonstrates high sensitivity and specificity, establishing it as a reliable independent marker for identifying plaque instability.
  • These findings support the use of ox-LDL as a prognostic tool to guide clinical decisions regarding CEA.