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Related Experiment Video

Updated: Jun 20, 2025

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment

Published on: March 2, 2016

Plate-Based High-Throughput Fluorescence Assay for Assessing Enveloped Virus Integrity.

Shannan-Leigh Macleod¹, Elana H Super¹, Lauren J Batt¹

¹Department of Materials and Henry Royce Institute, University of Manchester, Manchester M13 9PL, UK.

Biomacromolecules

|July 23, 2024

Summary

A new fluorescence assay (FAIRY) rapidly distinguishes infectious from noninfectious enveloped viruses. This high-throughput method is crucial for studying viruses and developing new antiviral therapies and vaccines.

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Last Updated: Jun 20, 2025

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Area of Science:

Virology
Biotechnology
Molecular Biology

Background:

Viruses pose significant global health and economic challenges.
Understanding viral infectivity is critical for developing vaccines and gene therapies.
Existing methods for assessing viral integrity are often slow or unavailable.

Purpose of the Study:

To develop a rapid, high-throughput assay for determining the infectivity of enveloped viruses.
To differentiate between intact (infectious) and nonintact (noninfectious) viruses.

Main Methods:

Development of a cell-free, plate-based assay named FAIRY (Fluorescence Assay for vIRal IntegritY).
Utilized a thiazole orange-terminated polymer to detect viral integrity.
Tested the assay's performance on various enveloped viruses subjected to heat or virucidal treatments.

Main Results:

The FAIRY assay demonstrated a 99% increase in fluorescence between treated and nontreated enveloped viruses.
Successfully differentiated intact from nonintact viruses, indicating infectious from noninfectious states.
Enabled rapid determination of infectivity for a range of enveloped viruses.

Conclusions:

The FAIRY assay is a robust and high-throughput tool for assessing enveloped virus infectivity.
This assay has significant potential for accelerating research on viruses and antiviral interventions.
FAIRY can aid in the quality control of viral-based therapeutics like vaccines and gene therapies.