Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Networks02:26

Protein Networks

3.9K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.9K
Protein-protein Interfaces02:04

Protein-protein Interfaces

12.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K
Proteomics01:33

Proteomics

7.3K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.3K
The Equilibrium Binding Constant and Binding Strength02:18

The Equilibrium Binding Constant and Binding Strength

12.9K
The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
12.9K
Ligand Binding Sites02:40

Ligand Binding Sites

12.8K
Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
12.8K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.1K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

WSO-YOLO11: a wavelet-strip-oriented YOLO11 framework for hot-rolled steel strip surface defect detection.

Scientific reports·2026
Same author

Targeting SMC3 deacetylation synergizes with XPO1 inhibition to reprogram the epigenetic landscape and suppress NPM1-mutated acute myeloid leukemia.

Nature communications·2026
Same author

A multi species autophagy-inducing nano-vaccine platform: uncovering immunoenhancement properties of PCV2 cap nanoparticles.

Journal of nanobiotechnology·2026
Same author

TH5487 specifically targets NLRP3 in FCAS patients resistant to MCC950.

Communications biology·2026
Same author

Correction: Association between EAT-Lancet diet adherence and incident type 2 diabetes in Shanghai adults: a prospective cohort study.

BMC public health·2026
Same author

Yifei Sanjie pill combined with gefitinib reduces the progression of EGFR-TKIs-resistant non-small cell lung cancer via YAP/ANKRD1 axis.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2026
Same journal

Decoding Viral Dark Matter: Metagenomic Prokaryotic Virus Characterization With Pharokka, Phold, and Phynteny.

Current protocols·2026
Same journal

A Novel Laboratorial Approach to Evaluate Bacterial Microleakage of Endodontic Sealers.

Current protocols·2026
Same journal

TRIAGE Toolkit: Streamlined Discovery of Regulatory Genes and Elements.

Current protocols·2026
Same journal

High-throughput Profiling of Pseudouridines in Microbiome-derived Bacterial RNA.

Current protocols·2026
Same journal

Recombinant Protein Expression in Rhodococcus species.

Current protocols·2026
Same journal

Streamlined In Vitro Transcription for Generating Self-Amplifying RNA With Modified Nucleotides.

Current protocols·2026
See all related articles

Related Experiment Video

Updated: Jun 20, 2025

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.1K

Measuring Interactions Between Proteins and Small Molecules or Nucleic Acids.

Angela Lackner1, Yanfei Qiu1, Emy Armanus1

  • 1Laboratory of Macromolecular Structure, Department of Molecular Biology' and Biochemistry, Charlie Dunlop School of Biological Sciences, University of California Irvine, Irvine, California.

Current Protocols
|July 23, 2024
PubMed
Summary
This summary is machine-generated.

This review highlights methods for studying protein interactions with small molecules and nucleic acids, crucial for understanding biological processes and developing new therapies. It covers both new and established techniques for detecting these vital molecular interactions.

Keywords:
disease researchdrug discoveryfluorescencemass specprotein interactions

More Related Videos

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
08:07

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry

Published on: January 17, 2025

1.3K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

3.5K

Related Experiment Videos

Last Updated: Jun 20, 2025

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST
10:28

Measuring Interactions of Globular and Filamentous Proteins by Nuclear Magnetic Resonance Spectroscopy NMR and Microscale Thermophoresis MST

Published on: November 2, 2018

12.1K
Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry
08:07

Analyzing DNA-Protein Interactions with Streptavidin-Based Biolayer Interferometry

Published on: January 17, 2025

1.3K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

3.5K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Drug Discovery

Background:

  • Protein interactions with small molecules and nucleic acids are fundamental to cellular functions and human health.
  • These interactions are key to processes like gene regulation, signal transduction, and metabolism.
  • Dysregulation of these interactions is implicated in various diseases.

Purpose of the Study:

  • To review and summarize state-of-the-art and established methods for detecting protein-small molecule interactions.
  • To provide an overview of techniques for studying protein-nucleic acid interactions.
  • To enhance understanding of molecular mechanisms and aid drug discovery.

Main Methods:

  • The review covers a wide array of detection methods with varying cost, sensitivity, and throughput.
  • It includes both novel techniques developed in the last decade and traditional methods.
  • Methods discussed are applicable to studying protein-small molecule and protein-nucleic acid binding.

Main Results:

  • A comprehensive overview of current and historical techniques for analyzing molecular interactions is presented.
  • The review emphasizes the importance of these methods for biological and medical research.
  • Newer techniques offer enhanced capabilities for studying these interactions.

Conclusions:

  • Understanding protein-small molecule and protein-nucleic acid interactions is critical for advancing biological sciences.
  • A diverse toolkit of methods is available, with ongoing development of new techniques.
  • This knowledge is essential for drug development and understanding disease.