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Nanopillar array-based electrochemical aptamer sensor for STX sensitivity detection
Jinsong Li1, Weixian Zheng2, Ya Gao1
1Anhui Provincial Engineering Center for High-Performance Biobased Nylons, Anhui Engineering Research Center for Highly Functional Fiber Products for Automobiles, School of Materials and Chemistry, Anhui Agricultural University, China. zbli@ahau.edu.cn.
A novel electrochemical aptasensor using ordered nanopillar arrays offers sensitive and selective detection of saxitoxin (STX). This method achieves a low limit of detection, crucial for monitoring shellfish poison.
Area of Science:
- Electrochemistry
- Nanotechnology
- Biosensing
Background:
- Saxitoxin (STX) is a highly toxic cyanotoxin requiring rapid detection methods.
- Existing detection techniques may lack sensitivity or selectivity for STX.
Purpose of the Study:
- To fabricate an ordered nanopillar array-based electrochemical aptasensor for high-performance STX detection.
- To achieve sensitive, selective, and facile STX detection for shellfish safety.
Main Methods:
- Fabrication of an Au@PAN nanopillar array electrode.
- Immobilization of methylene blue-labeled anti-STX aptamer (MB-Apt) as the recognition element.
- Electrochemical detection of STX using the developed aptasensor.
Main Results:
- The aptasensor demonstrated high sensitivity over wide linear ranges (1 pM-3 nM and 3-100 nM).
- Achieved a low limit of detection (LOD) of 1 pM for STX.
- Exhibited excellent selectivity against common interfering substances like neo-STX and metal ions.
Conclusions:
- The ordered nanopillar array strategy enhances electrochemical aptasensor performance for STX detection.
- The developed aptasensor is a promising tool for sensitive and selective monitoring of STX in shellfish.
- This approach offers a facile and effective method for developing advanced electrochemical sensors.
