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Hexagonal Plasmonic Arrays for High-Throughput Multicolor Single-Molecule Studies
Ediz Kaan Herkert1, Lukas Lau1, Roger Pons Lanau1
1ICFO - Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels, Barcelona, Spain.
ACS Applied Materials & Interfaces
|July 23, 2024
Summary
New hexagonal close-packed antenna-in-box (HCP-AiB) arrays enable high-throughput, multicolor single-molecule studies. This nanophotonic biosensor breakthrough overcomes previous limitations for advanced biomolecular analysis.
Area of Science:
- Nanophotonics
- Biosensing
- Single-molecule detection
Background:
- Nanophotonic biosensors enhance light for sensitive detection.
- Antenna-in-box (AiB) designs show promise but have limitations in multicolor studies and throughput.
- Current designs hinder correlative multicolor single-molecule analysis.
Purpose of the Study:
- To develop advanced nanophotonic biosensor arrays for high-throughput, multicolor single-molecule studies.
- To overcome limitations of existing antenna-in-box designs.
- To enable parallel readout and correlative multicolor analysis.
Main Methods:
- Introduction of aluminum-based hexagonal close-packed AiB (HCP-AiB) arrays.
- Parallel readout of over 1000 HCP-AiBs.
- Alternating three-color excitation scheme and epi-fluorescence detection.
- Use of optical fiducial markers for alignment-free studies.
Main Results:
- Achieved multicolor single-molecule sensitivity up to micromolar concentrations.
- Demonstrated high-throughput studies at micromolar and high single-molecule detection probabilities in the nanomolar range.
- Enabled robust, alignment-free correlative multicolor studies in the millisecond range.
Conclusions:
- HCP-AiB arrays overcome previous constraints in nanophotonic biosensing.
- These arrays facilitate high-throughput, multicolor single-molecule studies.
- Advancements pave the way for novel biosensor architectures for single-molecule dynamics research.
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