Lymphadenopathy Tissue Sampling by EUS-Guided Fine-Needle Biopsy Contributes to Meeting the Conditions for Genomic...

Area of Science:

• Gastroenterology
• Oncology
• Pathology

Background:

• Endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) using Franseen or Fork-tip needles enhances tissue acquisition.
• The utility of EUS-FNB for lymphadenopathy genomic profiling remains under investigation.

Purpose of the Study:

• To evaluate the efficacy of EUS-FNB versus EUS-fine needle aspiration (EUS-FNA) for tissue acquisition and genomic profiling in patients with lymphadenopathy.
• To compare the suitability of tissue obtained by EUS-FNB and EUS-FNA for molecular analysis.

Main Methods:

• Patients with abdominal lymphadenopathy underwent either EUS-FNA or EUS-FNB.
• Acquired tissue quantity and suitability for genomic profiling were assessed using pathologic adequacy scores and specific genomic profiling criteria (FoundationOne CDx, NCC Oncopanel).

Main Results:

• EUS-FNB yielded significantly higher pathologic adequacy scores and suitability for genomic profiling compared to EUS-FNA.
• Multivariate analysis identified EUS-FNB as the sole factor influencing genomic profiling suitability (OR 19.5, P <0.01).

Conclusions:

• EUS-FNB with Franseen or Fork-tip needles offers superior tissue acquisition for lymphadenopathy.
• This improved tissue yield enhances the suitability for genomic profiling, potentially improving personalized cancer treatment strategies.