Related Experiment Video
Updated: Jun 19, 2025
10:26
In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells
Published on: January 20, 2019
12.2K
Distinct epigenomic landscapes underlie tissue-specific memory T cell differentiation
Frank A Buquicchio1, Raissa Fonseca2, Patrick K Yan3
1Department of Pathology, Stanford University, Stanford, CA 94305, USA; Program in Immunology, Stanford University, Stanford, CA 94304, USA; Gladstone-UCSF Institute of Genomic Immunology, San Francisco, CA 94158, USA.
Immunity
|July 23, 2024
Summary
Tissue-resident memory T (TRM) cells and circulating memory T (TCIRC) cells exhibit distinct epigenetic landscapes. This study reveals key regulators and epigenetic signatures defining TRM cell development and function across organs.
Area of Science:
- Immunology
- Epigenetics
- Cell Biology
Background:
- Memory CD8+ T cell pool comprises heterogeneous subsets with specialized functions.
- Understanding the epigenetic basis of tissue-resident memory T (TRM) cell heterogeneity is crucial.
Purpose of the Study:
- To investigate the epigenetic landscape of memory CD8+ T cells in various non-lymphoid organs and circulation.
- To identify regulators and epigenetic signatures of tissue-resident memory T (TRM) cell development.
- To compare epigenetic states of TRM cells, circulating memory T (TCIRC) cells, and exhausted T (TEX) cells.
Main Methods:
- Single-cell transposase-accessible chromatin sequencing (scATAC-seq) applied to CD8+ T cells from seven non-lymphoid organs and circulation.
- Analysis of epigenetic landscapes across four distinct infection models.
- Identification of transcriptional regulators and epigenetic signatures.
Main Results:
- Tissue-resident memory T (TRM) cells and circulating memory T (TCIRC) cells follow distinct epigenetic trajectories.
- Organ-specific transcriptional regulators (FOSB, FOS, FOSL1, BACH2) and a common TRM epigenetic signature were identified.
- Terminal exhausted T (TEX) cells possess unique epigenetic features distinct from TRM cells.
Conclusions:
- Epigenetic reprogramming drives the development of distinct tissue-adapted memory CD8+ T cell states.
- Chromatin accessibility changes are critical for defining TRM cell specialization and function.
- This study provides a comprehensive epigenetic resource for understanding memory T cell heterogeneity.
Related Concept Videos
01:23Cells of the Adaptive Immune Response
975
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
975
03:17Inheritance of Chromatin Structures
6.2K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.2K
01:22T Cell Activation and Clonal Selection
694
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
694
01:23Immunological Memory
578
Immunological memory, a pivotal pillar of the adaptive immune system, is responsible for the body's ability to remember and respond more swiftly and effectively to previously encountered pathogens. This remarkable feature is what makes vaccines so effective in preventing diseases.
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...
578
01:32Chromatin Modification in iPS Cells
1.6K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.6K
01:20Special Features of Adaptive Immunity
786
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
786