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Updated: May 7, 2026

Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors
Published on: January 29, 2019
Production and Purification of Adeno-Associated Viral Vectors for the Development of Immune-Competent Mouse Models of
Lisa Schiller1, Chunkyu Ko2, Anna D Kosinska1,3
1Institute of Virology, Technical University of Munich, School of Medicine/Helmholtz Munich, Munich, Germany.
Abstract:
Mice infected with a recombinant adeno-associated virus carrying a replication-competent hepatitis B virus genome (rAAV-HBV) via the intravenous route establish a persistent HBV replication in hepatocytes and develop immune tolerance. They serve as models to evaluate antiviral immunity and to assess potential therapeutic approaches for chronic HBV infection. Combining selected HBV variants and different mouse genotypes allows for addressing a broad spectrum of research questions. This chapter describes the basic principles of the rAAV-HBV mouse model, rAAV-HBV production and purification methods, and finally, the in vivo application.

