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[A colloidal gold immunochromatography-based method for detecting enramycin residues in feed].
Summary
A new colloidal gold test strip enables rapid detection of enramycin in feed. This method, using an anti-enramycin A monoclonal antibody, offers high sensitivity and repeatability for large-scale feed analysis.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Food Safety
Background:
- Enramycin is an antibiotic used in animal feed.
- Rapid and sensitive detection methods for enramycin in feed are crucial for ensuring food safety and regulatory compliance.
- Existing methods like HPLC can be time-consuming and require specialized equipment.
Purpose of the Study:
- To develop a rapid, sensitive, and reliable colloidal gold immunochromatographic test strip for detecting enramycin A in feed samples.
- To optimize the test strip's performance by investigating key parameters such as pH, antibody titer, and antigen concentration.
- To evaluate the test strip's specificity, limit of detection, linear range, and repeatability compared to established methods.
Main Methods:
- Development of colloidal gold probes using a high-purity anti-enramycin A monoclonal antibody (anti-Er.A-mAb).
- Utilized a competitive inhibition method for the immunochromatographic test strip design.
- Optimized test strip parameters including pH, antibody concentration, and antigen concentration on the test line.
- Validated the test strip's performance using feed samples spiked with known enramycin concentrations.
Main Results:
- The optimized colloidal gold test strip demonstrated acceptable specificity and a low limit of detection (25 ng/mL) for enramycin A.
- A linear detection range of 25-300 ng/mL was established for enramycin A.
- The test strip exhibited good repeatability and higher sensitivity compared to high-performance liquid chromatography (HPLC).
Conclusions:
- The developed colloidal gold immunochromatographic test strip is suitable for the rapid and accurate detection of enramycin in large batches of feed samples.
- This method provides a sensitive and repeatable alternative to traditional analytical techniques for enramycin detection in feed.
- The test strip contributes to enhanced food safety monitoring and regulatory control in the animal feed industry.
