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Related Experiment Video

Updated: Jun 24, 2026

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
16:43

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Published on: February 18, 2014

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A naphthalimide-based fluorescent platform for endoplasmic reticulum targeted imaging.

Yiwei Li1,2, Nan Zhang1, Yan Wang1

  • 1Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China. xjliu@iccas.ac.cn.

Chemical Communications (Cambridge, England)
|July 24, 2024
PubMed
Summary

New naphthalimide dyes specifically target the endoplasmic reticulum (ER) due to lipophilic properties. Two dyes, TRNATR and TYNATY, effectively track ER stress and autophagy in living cells.

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Area of Science:

  • Organic Chemistry
  • Cell Biology
  • Biochemistry

Background:

  • The endoplasmic reticulum (ER) is a crucial organelle involved in protein folding and lipid synthesis.
  • ER stress can lead to various cellular dysfunctions and diseases.
  • Developing specific probes for ER visualization and stress monitoring is essential for biological research.

Purpose of the Study:

  • To design and synthesize novel naphthalimide-based fluorescent dyes.
  • To investigate the ER-targeting capabilities of these synthesized dyes.
  • To evaluate the potential of these dyes in tracking ER stress and autophagy in living cells.

Main Methods:

  • Synthesis of a series of naphthalimide derivatives with varied side chains.
  • Cellular imaging experiments to assess ER localization and specificity.
  • Live-cell imaging to monitor ER stress and autophagy induction using specific stimuli (tunicamycin, nutritional starvation).

Main Results:

  • Five naphthalimide dyes (TRNATR, MOTNAMOT, MPNAMP, TYNATY, PNAP) demonstrated specific targeting of the ER without requiring ER-targeting groups, attributed to their lipophilic properties.
  • The dyes TRNATR and TYNATY were successfully employed to study ER stress.
  • These two dyes effectively tracked ER autophagy in living cells under conditions of tunicamycin-induced stress and nutritional starvation.

Conclusions:

  • Novel naphthalimide dyes with inherent ER-targeting capabilities have been developed.
  • TRNATR and TYNATY are promising tools for real-time monitoring of ER stress and autophagy.
  • These findings offer new avenues for investigating ER-related cellular processes and diseases.