The development of a microimmunoadsorbent assay and a microdialysis method for the evaluation of immunoadsorbent...

A microimmunoadsorbent assay which provides for the quantitative elution and recovery of active antibody from immunoaffinity columns has been developed. We have also designed a microdialysis chamber which permits the dialysis of sample volumes from 50 to 500 microliters and allows quantitative recovery of macromolecular compounds. These techniques permit the rapid evaluation of a large number of eluants and immunoadsorbents in a short period of time. The microimmunoadsorbent assay coupled with the microdialysis method has been used to evaluate the efficacy of different anti-isopentenyl adenosine immunoadsorbents under various elution conditions. In the present study the best eluants for immunoaffinity purification of anti-N6-(delta 2-isopentenyl)adenosine antibody contained 15% pyridine in a sodium phosphate buffer or 4 M imidazole-HCl at pH 10.0.