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Design considerations and biomaterials selection in embedded extrusion 3D bioprinting
Swaprakash Yogeshwaran1, Hossein Goodarzi Hosseinabadi2,3, Daniel E Gendy1
1Department of Biomedical Engineering, Newark College of Engineering, New Jersey Institute of Technology, 323 Dr Martin Luther King Jr Blvd, Newark, NJ 07102, USA. am3296@njit.edu.
Biomaterials Science
|July 24, 2024
Summary
Embedded extrusion 3D bioprinting and Freeform Reversible Embedding of Suspended Hydrogels (FRESH) offer rapid, controlled manufacturing of cell-laden tissues. This study guides bioink selection and printing parameters for these advanced bioprinting techniques.
Area of Science:
- Biotechnology
- Materials Science
- Regenerative Medicine
Background:
- Embedded extrusion 3D bioprinting utilizes a temporary matrix to support extruding filaments.
- Freeform Reversible Embedding of Suspended Hydrogels (FRESH) is a specific granular sacrificial matrix technique.
- These methods enable faster and more precise fabrication of cell-laden tissue constructs, including vascular and multi-component structures.
Purpose of the Study:
- To detail the working principles of embedded extrusion and FRESH 3D bioprinting.
- To establish bioink design criteria for these bioprinting strategies.
- To provide experimental data for selecting appropriate support matrices and printing parameters.
Main Methods:
- Review of embedded extrusion and FRESH 3D bioprinting principles.
- Development of bioink design criteria.
- Presentation of experimental data for matrix selection.
- Discussion of advantages over conventional biomanufacturing.
Main Results:
- Guidance on selecting support baths or matrices for embedded extrusion and FRESH bioprinting.
- Insights into bioink formulation and printing parameter optimization.
- Demonstration of enhanced control and speed in fabricating complex tissue constructs.
Conclusions:
- Embedded extrusion and FRESH bioprinting are advanced techniques for rapid and controlled tissue engineering.
- Proper bioink selection and parameter optimization are crucial for successful fabrication.
- These methods present significant advantages over traditional biomanufacturing approaches for vascular and multi-component tissue constructs.
