Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electrodes in clinical chemistry.

A D Hirst, J F Stevens

    Annals of Clinical Biochemistry
    |September 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    For direct estimation, avoid calibrants adjusted to constant ionic strength. For indirect methods, calibrant adjustment likely has minimal impact, simplifying concentration calculations and activity estimations using specific algorithms.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Reducing gut microbiome-driven adipose tissue inflammation alleviates metabolic syndrome.

    Microbiome·2023
    Same author

    Practical guide to the Idea, Development and Exploration stages of the IDEAL Framework and Recommendations.

    The British journal of surgery·2016
    Same author

    Osmolality as a measure of dehydration in the neonatal period.

    Archives of disease in childhood·2010
    Same author

    Identification of isoflavones in the roots of Pueraria lobata.

    Planta medica·2007
    Same author

    CYP2K6 from zebrafish (Danio rerio): cloning, mapping, developmental/tissue expression, and aflatoxin B1 activation by baculovirus expressed enzyme.

    Comparative biochemistry and physiology. Toxicology & pharmacology : CBP·2005
    Same author

    Menstrual cycle effects on hypothalamic dopamine receptor function in women with a history of puerperal bipolar disorder.

    Journal of psychopharmacology (Oxford, England)·2003

    Area of Science:

    • Analytical Chemistry
    • Electrochemistry

    Background:

    • Accurate chemical analysis relies on appropriate calibration standards.
    • Ionic strength significantly influences ion activity and concentration measurements.
    • Direct vs. indirect methods present different calibration challenges.

    Purpose of the Study:

    • To theoretically evaluate the impact of ionic strength adjustment in calibrants for chemical analysis.
    • To determine optimal calibration strategies for direct and indirect measurement techniques.
    • To compare the influence of calibration procedures versus junction potential variations.

    Main Methods:

    • Theoretical analysis of calibration principles.
    • Development of algorithms for concentration and activity estimation.
    • Comparison of direct and indirect analytical methodologies.

    Related Experiment Videos

    Main Results:

    • Constant ionic strength adjustment is theoretically preferable for direct concentration or activity estimation.
    • For indirect methods using ionic strength adjusting buffers, adjusted and unadjusted calibrants yield similar results.
    • Direct concentration estimation algorithms are more complex than those for activity estimation.

    Conclusions:

    • Indirect methods are necessary for accurate concentration determination.
    • Direct methods are required for precise activity measurements.
    • Calibration procedure variations may have less quantitative impact than junction potential variations.