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Related Experiment Video

Updated: Jun 19, 2025

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents

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Presynaptic Rac1 in the hippocampus selectively regulates working memory.

Jaebin Kim¹, Edwin Bustamante¹, Peter Sotonyi²

¹Department of Cell Biology, Duke University School of Medicine, Durham, United States.

|July 24, 2024

Summary

Rac1 signaling impacts spatial working memory via presynaptic terminals and long-term memory via postsynaptic sites. This study uncovers Rac1

Keywords:

Rac1 cell biology hippocampus mouse neuroscience presynaptic short-term plasticity working memory

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Investigating Long-term Synaptic Plasticity in Interlamellar Hippocampus CA1 by Electrophysiological Field Recording

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Investigating Long-term Synaptic Plasticity in Interlamellar Hippocampus CA1 by Electrophysiological Field Recording

Investigating Long-term Synaptic Plasticity in Interlamellar Hippocampus CA1 by Electrophysiological Field Recording

Published on: August 11, 2019

Area of Science:

Neuroscience
Cell Biology
Molecular Biology

Background:

Rac1, a small GTPase, is involved in actin remodeling and signaling.
Rac1 signaling influences learning, memory, and synaptic plasticity.
The precise roles of Rac1 in different cognitive functions and plasticity remain unclear.

Purpose of the Study:

To investigate the distinct roles of Rac1 in presynaptic and postsynaptic compartments.
To elucidate the molecular mechanisms underlying presynaptic Rac1 function in cognition.
To identify proteins and post-translational modifications associated with presynaptic Rac1 signaling.

Main Methods:

Utilized a genetically encoded Rac1 inhibitor targeted to presynaptic terminals.
Employed mass spectrometry to analyze the presynaptic proteomic and post-translational landscape.
Examined changes in synaptic vesicle distribution, morphology, and postsynaptic ultrastructure.

Main Results:

Presynaptic Rac1 inhibition selectively impaired spatial working memory in mice.
Postsynaptic Rac1 inhibition affected longer-term cognitive processes.
Identified serine/threonine kinases and phosphorylated proteins regulating synaptic vesicles at active presynaptic Rac1 sites.
Observed alterations in synaptic vesicle dynamics and postsynaptic structures.

Conclusions:

Rac1 signaling plays a critical, previously unrecognized, presynaptic role in cognitive processes.
Distinct presynaptic and postsynaptic functions of Rac1 contribute to different forms of memory and plasticity.
Presynaptic Rac1 regulates synaptic vesicle function, impacting cognitive performance.