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An automated method for leucocyte adherence inhibition testing.

D K McLeod, W H Isbister

The Australian Journal of Experimental Biology and Medical Science

|June 1, 1985

Summary

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An automated assay was developed to improve the reproducibility of the leucocyte adherence inhibition test for detecting anti-tumour immunity. This new method shows promising results in preliminary clinical studies, correlating with patient status.

Area of Science:

Immunology
Cancer Research
Biomedical Engineering

Background:

The leucocyte adherence inhibition test, used since 1974 to detect anti-tumour cell-mediated immunity, has faced criticism for being unreproducible.
These reproducibility issues may stem from the complex washing procedure in the original test.

Purpose of the Study:

To develop an automated assay for the leucocyte adherence inhibition test to enhance reproducibility.
To enable concurrent testing of replicate samples for improved efficiency and reliability.

Main Methods:

An automated apparatus was designed using 30 extended syringes (100 µL barrels, 1500 µL cones) with synchronous plungers.
Peripheral blood leucocytes, serum, and tumour antigen were incubated in the syringes.
Non-adherent cells were removed by automated washing with saline, and the effluent was counted.

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Main Results:

Statistical analysis (ANOVA using SPSS) confirmed minimal variance between syringes, justifying control and test group subdivisions.
Despite prototype limitations causing minor variations in aspiration volumes, preliminary clinical studies demonstrated a correlation in 13 out of 15 patients with their Mantoux status.

Conclusions:

The developed automated assay offers a more reproducible method for detecting anti-tumour cell-mediated immunity compared to the original leucocyte adherence inhibition test.
Preliminary clinical data suggest the automated test's potential utility in assessing immune responses in cancer patients.