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An Integrated and Modular Compartmentalized Microfluidic System with Tunable Electrospun Porous Membranes for

Roa S Fardous1,2, Sultan Alshmmari3,2, Essam Tawfik4

  • 1Strathclyde Institute of Pharmacy and Biomedical Sciences, Strathclyde University, Glasgow G4 0RE, U.K.

ACS Applied Materials & Interfaces
|July 24, 2024
PubMed
Summary

We developed a novel 3D microfluidic system using electrospun porous membranes (PMs) for organ-on-a-chip applications. This integrated system allows for efficient drug permeability studies and in vitro modeling of epithelial barriers.

Keywords:
PMMAcell culturecompartmentalizedelectrospinningintegrationmicrofluidicsporous membrane

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Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Microfluidics

Background:

  • Organ-on-a-chip systems require advanced microfluidic devices with integrated porous membranes.
  • Current fabrication methods can be complex and require multiple assembly steps.

Purpose of the Study:

  • To present a modular, 3D compartmentalized microfluidic system with in situ electrospun porous membranes (PMs).
  • To demonstrate a novel, high-throughput fabrication method for integrated PMs in microfluidic chips.
  • To evaluate PMMA-based membranes for epithelial cell culture and drug permeability studies.

Main Methods:

  • Direct electrospinning of polymer nanofibers onto patterned poly(methyl methacrylate) (PMMA) substrates.
  • Fabrication of 3D compartmentalized microfluidic chips with integrated PMs.
  • Culturing Caco-2 epithelial cells to form a monolayer for drug permeability assessment.
  • Characterization of membrane properties, including fiber diameter, porosity, and mechanical integrity.

Main Results:

  • Successfully integrated electrospun PMs into various microfluidic chip designs using a novel in situ deposition method.
  • PMMA-based membranes (PMMA/PVP ratio 5:1) demonstrated uniform structure, tunable porosity, and excellent mechanical integrity.
  • The developed system enabled dynamic in vitro modeling of epithelial barrier function and drug transport.

Conclusions:

  • The novel electrospinning approach offers a streamlined, high-throughput method for fabricating integrated porous membranes in microfluidic devices.
  • The PMMA-PVP membranes provide a tunable platform for advanced organ-on-a-chip applications, including drug screening and disease modeling.
  • This technology facilitates dynamic in vitro studies of epithelial barriers for improved drug development and physiological research.