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Aptamer and sodium alginate decorated graphene oxide composite material with ion responsiveness for Low-density
Jianfang Cao1, Yuanshang Wang2, Linshi Jin3
1Institute of Food & Nutrition Science and Technology, Shandong Provincial Key Laboratory of Agro‑Products Processing Technology, Key Laboratory of Novel Food Resources Processing, Ministry of Agriculture, Shandong Academy of Agricultural Sciences, Jinan 250100, China.
Insights
Researchers developed a novel graphene oxide composite (GO@Apt@SA) for efficient Low-density lipoprotein (LDL) removal. This material demonstrates high adsorption capacity and specific LDL extraction from complex samples, offering a promising approach for cardiovascular health management.
Area of Science:
- Materials Science
- Biotechnology
- Analytical Chemistry
Background:
- Excess Low-density lipoprotein (LDL) is a key factor in cardiovascular diseases like heart failure and coronary artery disease.
- Current methods for decreasing LDL levels face ongoing challenges and require improvement.
Purpose of the Study:
- To develop an ionic stimulation-responsive composite for enhanced LDL adsorption and extraction.
- To investigate the adsorption capacity and specificity of the novel composite material.
Main Methods:
- Modification of graphene oxide (GO) with LDL-aptamer (Apt) and sodium alginate (SA) to create GO@Apt@SA.
- Evaluation of the composite's ion-responsive behavior and LDL adsorption capacity under optimal conditions.
- Assessment of LDL release triggered by an aptamer complementary chain and performance in goat serum.
Main Results:
- The GO@Apt@SA composite demonstrated synergistic ion-responsive behavior and aptamer-specific recognition for LDL.
- Maximum LDL adsorption capacity reached 730.6 μg mg⁻¹ under optimal experimental conditions.
- Successful LDL release was achieved using an aptamer complementary chain, and the material showed effective performance in goat serum.
Conclusions:
- The developed GO@Apt@SA composite offers high capacity and specificity for LDL adsorption.
- The material and technology are suitable for extracting LDL from complex biological matrices.
- This advancement holds potential for managing hypercholesterolemia and related cardiovascular conditions.
Abstract:
The accumulation of excess Low-density lipoprotein (LDL) is strongly associated with the occurrence of heart failure, coronary artery disease and hypercholesterolaemia, and is a major factor in cardiovascular and cerebrovascular disease. Concerns about the ways to decrease LDL level have continuously arisen. In this study, an ionic stimulation-responsive composite (i.e., GO@Apt@SA) is prepared with modification of graphene oxide (GO) utilising LDL-aptamer (Apt) and sodium alginate (SA). The ion-responsive behaviour of GO@Apt@SA synergistically interacts with the specific recognition property of the aptamer, enabling adsorption of LDL with higher capacity and specificity. Under the optimal experimental conditions, the maximum adsorption capacity of GO@Apt@SA for LDL is 730.6 μg mg-1. Interestingly, the aptamer complementary chain could trigger the release of LDL with favourable elution efficiency, which competitively binds with LDL-specific aptamer to trigger LDL release. More importantly, GO@Apt@SA exhibits satisfactory adsorption performances for LDL in goat serum, meaning that the composite material and technology are available for the extraction of LDL from complex sample matrices.
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