Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein-protein Interfaces02:04

Protein-protein Interfaces

12.5K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
12.5K
Protein-Drug Binding: Determination Methods01:22

Protein-Drug Binding: Determination Methods

156
Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
Indirect methods involve isolating the bound drug from its free form in biological samples such as blood, serum, or plasma. These techniques aim to measure the percentage of drugs bound to proteins. Equilibrium dialysis is a commonly used method where the free drug concentration at equilibrium is measured by separating the bound...
156
Protein Networks02:26

Protein Networks

3.9K
An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
3.9K
Structure-Activity Relationships and Drug Design01:28

Structure-Activity Relationships and Drug Design

694
Drug design is a dynamic field that involves discovering and developing new medications based on specific biological targets. This process heavily relies on structure-activity relationships (SAR) and quantitative structure-activity relationships (QSAR) to guide the design and optimization of efficient drugs.
SAR studies the intricate relationship between a drug's chemical structure and biological activity. It focuses on understanding how modifications to a drug's structure can influence...
694
Protein-Drug Binding: Mechanism and Kinetics01:16

Protein-Drug Binding: Mechanism and Kinetics

383
Protein-drug binding refers to the interaction between drugs and proteins within the body. This binding process can occur intracellularly, involving drug interactions with enzymes or receptors within cells, or extracellularly, involving plasma proteins in the blood.
Various forces drive these interactions, including hydrogen bonds, hydrophobic interactions, ionic bonds, electrostatic interactions, and van der Waals forces. These bonds enable drugs to bind to specific sites on proteins,...
383
Factors Affecting Protein-Drug Binding: Drug-Related Factors01:18

Factors Affecting Protein-Drug Binding: Drug-Related Factors

92
Drug binding to proteins is a complex phenomenon influenced by various drug-related factors, each playing a significant role in the interaction between drugs and proteins within the body.
One crucial factor in drug-protein binding is the drug's lipophilicity or its affinity for fat. More lipophilic drugs tend to have higher binding extents. For example, highly lipophilic drugs like cloxacillin exhibit substantial protein binding, with as much as 95% of the drug binding to proteins. In...
92

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Disaggregation Strategy for Nanopesticide Fabrication: Investigating the Impact of Nanosizing on Pesticide Biointeractions.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Single-cell analyses redefine cell heterogeneity signatures in diffuse large B-cell lymphoma and follicular lymphoma: Molecular insights and clinical impacts.

Journal of the Formosan Medical Association = Taiwan yi zhi·2026
Same author

The soil microbiome and metabolome in concert shape the flavor profile of ancient tea plants from Laowu mountain region.

Frontiers in plant science·2026
Same author

Metabolic symbiosis and competition: the dual nature of TAM-tumor cell cross-talk in tumor progression.

Frontiers in oncology·2026
Same author

ER-Negative Endometrial Cancers: An Evolving Diagnostic Category with Major Clinical Implications.

Cancers·2026
Same author

A prediction model for admission to the intensive care unit in patients with perianal necrotizing fasciitis.

Journal of infection in developing countries·2025

Related Experiment Video

Updated: Jun 19, 2025

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.0K

Emerging affinity methods for protein-drug interaction analysis.

Xinxin Zheng1, Huiting Zhu1, Xue Zhao1

  • 1Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, Shaanxi 710069, China.

Journal of Pharmaceutical and Biomedical Analysis
|July 24, 2024
PubMed
Summary

This review highlights advanced protein-drug interaction research methods. Emerging technologies enhance sensitivity and accuracy for drug development and disease treatment.

Keywords:
Affinity chromatographyAffinity magnetic levitationBiosensorIn-cell nuclear magnetic resonanceNative mass spectrometryProtein-drug interaction

More Related Videos

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation
14:44

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Published on: September 24, 2012

20.5K
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
08:23

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Published on: August 6, 2018

11.3K

Related Experiment Videos

Last Updated: Jun 19, 2025

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.0K
A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation
14:44

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Published on: September 24, 2012

20.5K
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
08:23

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Published on: August 6, 2018

11.3K

Area of Science:

  • Biochemistry
  • Pharmacology
  • Analytical Chemistry

Background:

  • Protein-drug interactions are vital for drug discovery and understanding disease mechanisms.
  • Traditional methods have limitations in sensitivity and scope.
  • Advancements in analytical technologies are revolutionizing the field.

Purpose of the Study:

  • To review emerging research methods for studying protein-drug interactions.
  • To discuss the principles, applications, advantages, and limitations of these novel technologies.
  • To highlight progress in establishing drug-protein interaction networks.

Main Methods:

  • Native mass spectrometry
  • Infrared spectroscopy
  • Nuclear magnetic resonance (NMR) and spectrum
  • Biosensor technologies (surface-enhanced Raman, electrochemistry, magneto-resistive signals)
  • Affinity magnetic levitation
  • Affinity chromatography

Main Results:

  • Significant progress in analytical method sensitivity, precision, and accuracy.
  • Enabling the establishment of comprehensive drug-protein interaction networks.
  • Demonstrating the broad applicability of these advanced techniques.

Conclusions:

  • Emerging technologies offer powerful tools for protein-drug interaction analysis.
  • These methods are crucial for accelerating drug development and improving disease treatment strategies.
  • Continued innovation in analytical techniques will further advance the field.