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  11. Amentoflavone Regulates The Mir-124-3p/capn2 Axis To Promote Mitochondrial Autophagy In Hcc Cells.

Amentoflavone regulates the miR-124-3p/CAPN2 axis to promote mitochondrial autophagy in HCC cells.

Fengting Zhu1, Jingwen Jiang1, Xuewu Chen1

  • 1Department of Oncology, Guangdong Provincial Hospital of Traditional Chinese Medicine Hainan Hospital, No. 13, Shunda Road, Meilan District, Haikou 570203, Hainan Province, P.R. China.

Toxicology Research
|July 25, 2024

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View abstract on PubMed

Summary
This summary is machine-generated.

Amentoflavone (AF) inhibits hepatocellular carcinoma (HCC) by regulating miR-124-3p and CAPN2, promoting mitochondrial autophagy and inhibiting the Wnt/β-catenin pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Hepatocellular carcinoma (HCC) presents a significant challenge due to its poor prognosis and high mortality.
  • Amentoflavone (AF) demonstrates potential as an anti-HCC agent with low toxicity and stable efficacy.

Purpose of the Study:

  • To elucidate the molecular mechanisms by which Amentoflavone (AF) inhibits HCC progression.
  • To explore the potential of AF as a novel therapeutic strategy for HCC treatment.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and Western blot were used to assess gene and protein expression.
  • Cell viability was determined using the CCK-8 assay.
  • Dual-luciferase reporter assays confirmed the interaction between miR-124-3p and CAPN2.
Keywords:
AmentoflavoneCAPN2hepatocellular carcinomamiR-124-3p

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Main Results:

  • AF treatment decreased HCC cell viability and promoted mitochondrial autophagy.
  • AF upregulated miR-124-3p expression while downregulating CAPN2 and inhibiting β-catenin nuclear translocation.
  • The miR-124-3p/CAPN2 axis was identified as a key regulator in AF's anti-HCC effects.

Conclusions:

  • Amentoflavone (AF) exerts anti-HCC effects by modulating the miR-124-3p/CAPN2 axis.
  • This regulation inhibits β-catenin nuclear translocation and the Wnt/β-catenin pathway, ultimately promoting mitochondrial autophagy in HCC cells.
mitochondrial autophagy