Related Experiment Video
Updated: Jun 19, 2025
Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
Published on: January 31, 2018
Evaluation of Near-Infrared Laser Effects on 143B Cells: A System Biology Approach
Mohammad Rostami Nejad1, Masoumeh Farahani2, Zahra Razzaghi3
1Celiac Disease and Gluten Related Disorders Research Center, Research Institute for Gastroenterology and Liver Disease, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract:
Introduction: Photothermal therapy (PTT) by using a near-infrared (NIR) laser, as a successful treatment of cancer, has attracted extensive attention of researchers. Its advantages as a noninvasive and suitable method have been confirmed. Discovery of the NIR laser molecular mechanism at the cellular level via system biology assessment to identify the crucial targeted genes is the aim of this study. Methods: RNA-seq series of six samples were retrieved from Gene Expression Omnibus (GEO) and pre-evaluated by the GEO2R program for more analysis. The significant differentially expressed genes (DEGs) were determined and studied via gene expression analysis, protein-protein interaction (PPI) network assessment, action map evaluation, and gene ontology enrichment. Results: HSPA5, DDIT3, TRIB3, PTGS2, HMOX1, ASNS, GDF15, SLC7A11, and SQSTM1 were identified as central genes. Comparing the central genes and the determined crucial genes via gene expression analysis, actin map results, and gene ontology enrichment led to the introduction of HSPA5, DDIT3, PTGS2, HMOX1, and GDF15 as critical genes in response to the NIR laser. Conclusion: The results indicated that the principle biological process "Endoplasmic reticulum unfolded protein response" and HSPA5, DDIT3, PTGS2, HMOX1, and GDF15 are the crucial targets of the NIR laser. The results also showed that the NIR laser induces stress conditions in the irradiated cells.
Insights
This study identifies key genes like HSPA5 and DDIT3 involved in the cellular response to near-infrared (NIR) laser photothermal therapy (PTT). These findings reveal crucial molecular targets for optimizing this noninvasive cancer treatment.
Area of Science:
- Biomedical Engineering
- Molecular Biology
- Cancer Research
Background:
- Photothermal therapy (PTT) using near-infrared (NIR) lasers is a promising noninvasive cancer treatment.
- Understanding the molecular mechanisms of NIR laser action at the cellular level is crucial for optimizing PTT efficacy.
- System biology approaches can identify key genes and pathways affected by PTT.
Purpose of the Study:
- To elucidate the molecular mechanisms of NIR laser-induced cellular responses.
- To identify crucial targeted genes and biological processes affected by NIR laser PTT.
- To discover potential therapeutic targets for enhancing PTT outcomes in cancer treatment.
Main Methods:
- Utilized RNA-sequencing data from Gene Expression Omnibus (GEO) for analysis.
- Employed GEO2R for differential gene expression analysis.
- Conducted protein-protein interaction network assessment, action map evaluation, and gene ontology enrichment analysis.
Main Results:
- Identified nine central genes, including HSPA5, DDIT3, and HMOX1.
- Determined HSPA5, DDIT3, PTGS2, HMOX1, and GDF15 as critical genes in response to NIR laser irradiation.
- Highlighted the "Endoplasmic reticulum unfolded protein response" as a key biological process.
Conclusions:
- HSPA5, DDIT3, PTGS2, HMOX1, and GDF15 are identified as crucial molecular targets of NIR laser PTT.
- The "Endoplasmic reticulum unfolded protein response" is a principal biological process modulated by NIR laser therapy.
- NIR laser irradiation induces cellular stress conditions, providing insights into PTT's therapeutic effects.
More Related Videos
10:17Laser-induced Breakdown Spectroscopy: A New Approach for Nanoparticle's Mapping and Quantification in Organ Tissue
Published on: June 18, 2014
13:38Laser-Induced Fluorescence Emission L.I.F.E. as Novel Non-Invasive Tool for In-Situ Measurements of Biomarkers in Cryospheric Habitats
Published on: October 26, 2019