Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

SNAREs and Membrane Fusion01:43

SNAREs and Membrane Fusion

10.8K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
10.8K
Mechanisms of Membrane-bending01:15

Mechanisms of Membrane-bending

2.7K
The living membranes are flexible due to their fluid mosaic nature; however, their bending into different shapes is an active process regulated by specific lipids and proteins. The membrane bending can be transient as seen in vesicles or stable for a long time as in microvilli. Cells regulate the size, location, and duration of the membrane curvature.
Membrane bending can happen due to intrinsic changes in lipid composition or extrinsic association with different proteins. The proteins involved...
2.7K
Membrane Asymmetry Regulating Transporters01:19

Membrane Asymmetry Regulating Transporters

4.3K
Enzymes like flippase, floppase, and scramblase transfer phospholipids from one layer to another in the membrane, thereby affecting membrane asymmetry.
Flippase
Eukaryotic flippases are type-IV P-type ATPases or P4-ATPases belonging to P-type ATPase family proteins that are membrane-bound pumps involved in the ATP-mediated transport of ions and molecules across the membrane. Flippases flip specific phospholipids from the outer to the inner leaflet of a membrane. All P4-ATPases have one...
4.3K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.7K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
4.7K
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

5.7K
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
5.7K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.6K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Advancing physical virology through multiscale approaches-Insights from the 2025 FEBS|EMBO lecture course 'Physical Virology: across length scales'.

FEBS letters·2026
Same author

Biophysical approaches for studying viral entry.

FEBS letters·2026
Same author

A versatile GPMV-imaging platform for quantitative analysis of receptor binding and membrane fusion.

Biophysical journal·2025
Same author

Fusion of asymmetric membranes: the emergence of a preferred direction.

Faraday discussions·2025
Same author

Modulating the Curvature of Protein Self-Assembled Spiral Nanotubules.

ACS applied materials & interfaces·2025
Same author

Host Immune Cell Membrane Deformability Governs the Uptake Route of Malaria-Derived Extracellular Vesicles.

ACS nano·2025

Related Experiment Video

Updated: Jun 19, 2025

Nanopodia - Thin, Fragile Membrane Projections with Roles in Cell Movement and Intercellular Interactions
10:50

Nanopodia - Thin, Fragile Membrane Projections with Roles in Cell Movement and Intercellular Interactions

Published on: April 3, 2014

10.3K

Tetraspanin proteins in membrane remodeling processes.

Raviv Dharan1,2, Raya Sorkin1,2

  • 1School of Chemistry , Raymond & Beverly Sackler Faculty of Exact Sciences , Tel Aviv University, 6997801, Tel Aviv, Israel.

Journal of Cell Science
|July 25, 2024
PubMed
Summary

Tetraspanins (TSPANs) are key transmembrane proteins that remodel cell membranes. This review explores how TSPANs

Keywords:
Membrane biophysicsMembrane curvatureMembrane dynamicsMembrane remodelingTetraspaninTetraspanin-enriched microdomains

More Related Videos

Reconstitution of Msp1 Extraction Activity with Fully Purified Components
05:52

Reconstitution of Msp1 Extraction Activity with Fully Purified Components

Published on: August 10, 2021

2.4K
Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo
10:53

Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo

Published on: November 7, 2013

13.7K

Related Experiment Videos

Last Updated: Jun 19, 2025

Nanopodia - Thin, Fragile Membrane Projections with Roles in Cell Movement and Intercellular Interactions
10:50

Nanopodia - Thin, Fragile Membrane Projections with Roles in Cell Movement and Intercellular Interactions

Published on: April 3, 2014

10.3K
Reconstitution of Msp1 Extraction Activity with Fully Purified Components
05:52

Reconstitution of Msp1 Extraction Activity with Fully Purified Components

Published on: August 10, 2021

2.4K
Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo
10:53

Membrane-SPINE: A Biochemical Tool to Identify Protein-protein Interactions of Membrane Proteins In Vivo

Published on: November 7, 2013

13.7K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Membrane Biology

Background:

  • Membrane remodeling is vital for cellular functions like signaling, fusion, and migration.
  • Tetraspanins (TSPANs) are transmembrane proteins critical for these remodeling events.
  • TSPANs interact with other molecules, but their precise role in membrane dynamics is unclear.

Purpose of the Study:

  • To review recent findings on TSPANs in membrane remodeling.
  • To highlight TSPAN structural features enabling interactions and localization.
  • To emphasize the impact of membrane properties on TSPAN behavior and cellular functions.

Main Methods:

  • Literature review of recent studies on Tetraspanins and membrane remodeling.
  • Analysis of TSPAN structural characteristics and their role in molecular interactions.
  • Examination of the influence of membrane properties (curvature, tension, rigidity) on TSPANs.

Main Results:

  • TSPANs are central to membrane remodeling, interacting with various cellular components.
  • Membrane properties significantly influence TSPAN distribution and the formation of membrane domains.
  • TSPAN localization and interactions are modulated by membrane curvature and other physical properties.

Conclusions:

  • TSPANs play a multifaceted role in membrane remodeling, influenced by membrane biophysics.
  • Understanding TSPANs' structural features and membrane interactions is key to deciphering their function.
  • This review offers insights into the molecular mechanisms governing cellular membrane dynamics mediated by TSPANs.