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Updated: Jun 19, 2025

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity
Published on: March 25, 2020
RNase R vs. PNPase: selecting the best-suited exoribonuclease for environmental adaptation
1Department of Microbiology and Molecular Genetics, and Department of Molecular and Cellular Biology, University of California, Davis, CA, 95616, USA. pavan@ucdavis.edu.
Abstract:
3' → 5' exoribonucleases play a critical role in many aspects of RNA metabolism. RNase R, PNPase, and RNase II are the major contributors to RNA processing, maturation, and quality control in bacteria. Bacteria don't seem to have dedicated RNA degradation machineries to process different classes of RNAs. Under different environmental and physiological conditions, their roles can be redundant and sometimes overlapping. Here, I discuss why PNPase and RNase R may have switched their physiological roles in some bacterial species to adapt to environmental conditions, despite being biochemically distinct exoribonucleases.
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