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Updated: Jun 19, 2025

Identification of Kinase-substrate Pairs Using High Throughput Screening
Published on: August 29, 2015
Systematic Identification of Kinase-Substrate Relationship by Integrated Phosphoproteome and Interactome Analysis
Satoshi Muraoka1, Jun Adachi2,3
1Laboratory of Proteomics for Drug Discovery, Center for Drug Design Research, National Institute of Biomedical Innovation, Osaka, Japan.
None:
The sensitivity of phosphorylation site identification by mass spectrometry (MS)-based phosphoproteomics has improved significantly. However, the lack of kinase-substrate relationship (KSR) data has hindered improvement of the range and accuracy of kinase activity prediction using phosphoproteome data. We herein describe the application of a systematic identification of KSR by integrated phosphoproteome and interactome analysis using doxycycline (Dox)-induced target kinase-overexpressing HEK-293 cells.
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