Rapidly Inducible Yeast Surface Display for Antibody Evolution with OrthoRep

Area of Science:

• Biotechnology
• Molecular Biology
• Immunology

Background:

• Yeast surface display is a powerful tool for antibody engineering.
• The Autonomous Hypermutation Yeast Surface Display (AHEAD) platform rapidly generates potent antibodies.
• Current AHEAD systems rely on galactose induction, which can be slow (up to 48 hours).

Purpose of the Study:

• To enhance the AHEAD platform for faster antibody evolution.
• To investigate a novel synthetic β-estradiol-inducible system for antibody surface display.
• To improve the efficiency of antibody generation using yeast surface display.

Main Methods:

• Implementation of a synthetic β-estradiol-inducible gene expression system.
• Comparison of induction times between the new system and traditional galactose induction.
• Evaluation of the updated AHEAD platform in antibody evolution experiments.

Main Results:

• The synthetic β-estradiol-inducible system significantly accelerated antibody surface display.
• Faster induction was observed for both the updated AHEAD system and traditional yeast surface display.
• The updated AHEAD platform successfully drove rapid antibody evolution through multiple rounds.

Conclusions:

• A synthetic β-estradiol-inducible system offers a faster alternative for antibody display in yeast.
• This improvement enhances the AHEAD platform's efficiency for rapid antibody evolution.
• The updated AHEAD technology accelerates the generation of potent and specific antibodies.