Thermo-Switchable Enzyme@Metal-Organic Framework for Selective Biocatalysis and Biosensing.
Jiale Lin1, Cai Shen2, Yongfa Cheng1
1Zhejiang-Malaysia Joint Research Laboratory for Agricultural Product Processing and Nutrition, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, China.
ACS Applied Materials & Interfaces
|July 25, 2024
Summary
This study introduces a thermo-switchable enzyme@MOF (CRL@ZIF-8-PNIPAM) for size-selective biocatalysis. Temperature changes reversibly control pore size, enabling tunable enzyme selectivity for small and large molecules.
Area of Science:
- Biochemistry
- Materials Science
- Nanotechnology
Background:
- Enzyme immobilization enhances stability and reusability.
- Stimulus-responsive materials offer tunable control over enzyme activity.
- Metal-Organic Frameworks (MOFs) provide versatile platforms for enzyme encapsulation.
Purpose of the Study:
- To design and synthesize a thermo-switchable enzyme@MOF (CRL@ZIF-8-PNIPAM) for size-selective biocatalysis and biosensing.
- To investigate the temperature-dependent modulation of substrate selectivity in immobilized lipase.
- To develop an electrochemical biosensor utilizing the thermo-switchable enzyme@MOF for selective pesticide detection.
Main Methods:
- Immobilization of *Candida rugosa* lipase (CRL) within ZIF-8 functionalized with poly(*N*-isopropylacrylamide) (PNIPAM).
- Characterization of ZIF-8-PNIPAM nanostructures and their pore size modulation via temperature changes.
- Assay of hydrolytic activity for small (p-nitrophenyl butyrate) and large (p-nitrophenyl palmitate) substrates at different temperatures.
- Fabrication and testing of electrochemical biosensors for pesticide detection.
Main Results:
- CRL@ZIF-8-PNIPAM exhibited size-selective hydrolysis, favoring small molecules over large ones.
- The enzyme@MOF demonstrated thermo-switchable behavior, with significantly lower activity for large molecules at 40 °C (blocked pores) compared to 27 °C (open pores).
- A gradual pore size transition led to a delay in the thermo-switchable catalytic response during thermal cycling.
- Successful application in electrochemical biosensors for selective detection of pesticides based on molecular size.
Conclusions:
- The developed thermo-switchable enzyme@MOF enables tunable size-selective biocatalysis and biosensing.
- Temperature stimulus effectively modulates the pore size of ZIF-8-PNIPAM, controlling enzyme accessibility and selectivity.
- This platform holds promise for advanced applications in enzyme engineering and chemical sensing.
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