Dual-responsive electrochemical immunosensor for CYFRA21-1 detection based on Au/Co Co-loaded 3D ordered macroporous carbon interconnected framework

Qi Shen ¹, Jianjun Ding ¹, Zengsheng Guo ¹

⁰School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250055, PR China.

Colloids and Surfaces. B, Biointerfaces +

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July 25, 2024

View abstract on PubMed

Summary

A novel electrochemical immunosensor was developed to accurately detect cytokeratin-19 fragment antigen 21-1 (CYFRA21-1), a key biomarker for non-small cell lung cancer. This advanced sensor utilizes a unique 3D carbon structure with gold-cobalt nanoparticles for enhanced sensitivity and reliable detection in human serum.

Area Of Science

Electrochemistry
Nanomaterials Science
Biomedical Engineering

Background

Cytokeratin 19 fragment antigen 21-1 (CYFRA21-1) is a crucial biomarker for non-small cell lung cancer (NSCLC).
Accurate and sensitive detection of CYFRA21-1 is vital for early diagnosis and patient management.
Existing detection methods may face limitations in sensitivity or specificity.

Purpose Of The Study

To develop a novel, label-free electrochemical immunosensor for the sensitive and accurate detection of CYFRA21-1.
To utilize a unique 3D ordered interconnecting macroporous carbon skeleton material modified with gold-cobalt nanoparticles (Au/Co NPs-3D MCF).
To evaluate the performance of the developed immunosensor in detecting CYFRA21-1 in human serum.

Main Methods

Fabrication of a 3D ordered interconnecting macroporous carbon skeleton.
Modification of the carbon skeleton with gold-cobalt nanoparticles (Au/Co NPs).
Immobilization of antibodies for CYFRA21-1 detection and electrochemical analysis using chronocurrent testing and square-wave voltammetry.

Main Results

The Au/Co NPs-3D MCF immunosensor demonstrated a high specific surface area and enhanced electrochemical activity.
Achieved a wide linear response range from 0.0001-100 ng/mL for CYFRA21-1 detection.
Exhibited a low limit of detection and successful detection of CYFRA21-1 in human serum samples.

Conclusions

The developed dual-responsive label-free electrochemical immunosensor offers high sensitivity and selectivity for CYFRA21-1 detection.
The unique 3D nanostructure and synergistic effects of Au/Co NPs significantly enhance sensor performance.
This platform holds potential for early NSCLC diagnosis and can be extended for multiplexed biomarker detection.

Keywords:

3D ordered macroporous carbon interconnected framework Cytokeratin 19 fragment antigen 21–1 Dual-responsive Electrochemical immunosensor