Dual-responsive electrochemical immunosensor for CYFRA21-1 detection based on Au/Co Co-loaded 3D ordered macroporous...

Area of Science:

• Electrochemistry
• Nanomaterials Science
• Biomedical Engineering

Background:

• Cytokeratin 19 fragment antigen 21-1 (CYFRA21-1) is a crucial biomarker for non-small cell lung cancer (NSCLC).
• Accurate and sensitive detection of CYFRA21-1 is vital for early diagnosis and patient management.
• Existing detection methods may face limitations in sensitivity or specificity.

Purpose of the Study:

• To develop a novel, label-free electrochemical immunosensor for the sensitive and accurate detection of CYFRA21-1.
• To utilize a unique 3D ordered interconnecting macroporous carbon skeleton material modified with gold-cobalt nanoparticles (Au/Co NPs-3D MCF).
• To evaluate the performance of the developed immunosensor in detecting CYFRA21-1 in human serum.

Main Methods:

• Fabrication of a 3D ordered interconnecting macroporous carbon skeleton.
• Modification of the carbon skeleton with gold-cobalt nanoparticles (Au/Co NPs).
• Immobilization of antibodies for CYFRA21-1 detection and electrochemical analysis using chronocurrent testing and square-wave voltammetry.

Main Results:

• The Au/Co NPs-3D MCF immunosensor demonstrated a high specific surface area and enhanced electrochemical activity.
• Achieved a wide linear response range from 0.0001-100 ng/mL for CYFRA21-1 detection.
• Exhibited a low limit of detection and successful detection of CYFRA21-1 in human serum samples.

Conclusions:

• The developed dual-responsive label-free electrochemical immunosensor offers high sensitivity and selectivity for CYFRA21-1 detection.
• The unique 3D nanostructure and synergistic effects of Au/Co NPs significantly enhance sensor performance.
• This platform holds potential for early NSCLC diagnosis and can be extended for multiplexed biomarker detection.