A nanosilica-coated thread-based analytical device for nitrate and nitrite detection in food samples

A new microfluidic thread-based analytical device (μTAD) for nitrate and nitrite determination in food samples was developed. The cotton thread substrate was coated with nanosilica to increase its hydrophilicity and stability, and polylactic acid was applied to one end of the nanosilica-coated thread to constrain the fluid flow along the thread in one direction. Quantification of nitrate and nitrite was based on the modified Griess reaction, using sulfanilamide and N-(1-naphthyl) ethylenediamine as chromogenic reagents, and utilizing a distance-based detection technique. Linear responses were observed in a range of 4-25 mg L^-1 (R² = 0.9991) for nitrite and a range of 8-50 mg L^-1 (R² = 0.9989) for nitrate. The limits of detection for nitrite and nitrate were 1.5 and 3.1 mg L^-1, respectively. The detection time was 5 min for nitrite analysis, and 7 min for nitrate analysis. The new method demonstrated good precision, accuracy, selectivity, and stability. The performance of the proposed μTAD for nitrite and nitrate determination in real food samples was comparable to that of the conventional UV-Vis spectrophotometric method. The proposed μTAD could serve as a simple, low-cost, and portable method for nitrite and nitrate detection in food samples.