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Glutamine metabolism in fluorosis: Integrated metabolomics and transcriptomics analysis
Yue Ba1, Shu Niu1, Zichen Feng1
1Department of Environmental Health, School of Public Health, Zhengzhou University, 100 Kexue Avenue, Zhengzhou 450001, China.
The Science of the Total Environment
|July 25, 2024
Summary
This study identifies L-glutamine as a potential biomarker for fluorosis, a condition linked to altered glutamine metabolism. These findings offer new insights into fluorosis pathogenesis and diagnosis.
Area of Science:
- Metabolomics
- Biomarker Discovery
- Toxicology
Background:
- Fluorosis is a public health concern.
- Understanding its metabolic basis is crucial for diagnosis and treatment.
Purpose of the Study:
- To identify metabolic biomarkers for fluorosis.
- To elucidate the pathogenesis of fluorosis at a metabolic level.
Main Methods:
- Utilized GC-MS to analyze plasma metabolites in Sprague Dawley rats exposed to fluoride.
- Employed bioinformatics analyses including KEGG pathway enrichment and ROC analysis.
- Validated findings using transcriptomic data (GSE70719) and in vitro experiments.
Main Results:
- Identified significant differential metabolites (DMs) in fluoride-exposed rats compared to controls.
- L-glutamine demonstrated excellent diagnostic ability for fluorosis (AUC > 0.85).
- Glutamine metabolism pathways were significantly enriched, and GLS gene expression was altered.
Conclusions:
- L-glutamine is a promising metabolic biomarker for fluorosis.
- Glutamine metabolism plays a key role in the pathogenesis of fluorosis.
