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Updated: Jul 10, 2026

Techniques to Induce and Quantify Cellular Senescence
Published on: May 1, 2017
Brief guide to senescence assays using cultured mammalian cells
Eunseok Kang1, Chanhee Kang2, Young-Sam Lee3
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, South Korea.
Abstract:
Cellular senescence is a crucial biological process associated with organismal aging and many chronic diseases. Here, we present a brief guide to mammalian senescence assays, including the measurement of cell cycle arrest, change in cellular morphology, senescence-associated β-galactosidase (SA-β-gal) staining, and the expression of senescence-associated secretory phenotype (SASP). This work will be useful for biologists with minimum expertise in cellular senescence assays.

