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Human epidermal plasminogen activator. Characterization, localization, and modulation.

S Morioka, P J Jensen, G S Lazarus

    Experimental Cell Research
    |December 1, 1985
    PubMed
    Summary
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    Human epidermal cells express urokinase-type plasminogen activator (uPA), particularly during cell movement and differentiation. This enzyme

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Dermatology

    Background:

    • Plasminogen activator (PA) plays a role in extracellular matrix remodeling.
    • Understanding PA in human epidermal cells is crucial for skin biology.

    Purpose of the Study:

    • To investigate the type and localization of plasminogen activator (PA) in primary human epidermal cell cultures.
    • To determine the regulation of PA expression in relation to keratinocyte differentiation and migration.

    Main Methods:

    • Biochemical assays to measure PA activity.
    • Immunocytochemical staining using antibodies against urokinase.
    • Immunoblot and double immunodiffusion analyses.

    Main Results:

    • Human epidermal cells possess urokinase-type plasminogen activator (uPA).

    Related Experiment Videos

  • uPA activity was significantly inhibited by anti-urokinase antibody (>96%).
  • uPA staining was observed in migrating keratinocytes at colony edges and in differentiated cells.

    • Conclusions:

    • Epidermal PA is predominantly of the urokinase type.
    • uPA expression is regulated by cellular differentiation and cell movement in keratinocytes.
    • PA is involved in processes like wound healing and epidermal remodeling.