Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

6.6K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scaling covalent ligand discovery through dynamic combinatorial library-versus-proteome screening.

Nature communications·2026
Same author

Comparative Interactome Profiling of Itaconate and α-Ketoglutarate by the Peptide-Centric Local Stability Assay.

ACS chemical biology·2026
Same author

Identifying PLAU as a shared pathogenic gene in type 2 diabetes and bladder urothelial carcinoma through integrated transcriptomic analysis and machine learning for diagnostic and therapeutic value.

Clinical and experimental medicine·2026
Same author

Ligand-modulated metal-radical polarity match enables general 1,2-dicarbofunctionalization of ethylene.

Nature chemistry·2026
Same author

Resting-state brain network alterations in adolescent idiopathic scoliosis using functional near-infrared spectroscopy.

Biomedical engineering online·2026
Same author

Clinical outcomes and prognostic factors of systemic therapy combined with interventional treatment in hepatocellular carcinoma: a multicenter retrospective case series.

Translational cancer research·2026

Related Experiment Video

Updated: Jun 19, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.9K

Amplifiable protein identification via residue-resolved barcoding and composition code counting.

Weiming Guo1, Yuan Liu1, Yu Han1

  • 1Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China.

National Science Review
|July 26, 2024
PubMed
Summary
This summary is machine-generated.

This study introduces AmproCode, a novel method for protein identification. It uses DNA barcoding to create amplifiable protein fingerprints, enabling ultrasensitive detection and paving the way for next-generation protein sequencing.

Keywords:
DNA barcodingamplifiable fingerprintingcomposition code countingprotein identificationresidue-specific chemistry

More Related Videos

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
14:58

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

Published on: November 12, 2012

48.3K
An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

3.4K

Related Experiment Videos

Last Updated: Jun 19, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
10:37

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

11.9K
Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
14:58

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry

Published on: November 12, 2012

48.3K
An Integrated Approach for Microprotein Identification and Sequence Analysis
09:37

An Integrated Approach for Microprotein Identification and Sequence Analysis

Published on: July 12, 2022

3.4K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Proteomics

Background:

  • Ultrasensitive protein identification is crucial for research and diagnostics.
  • Direct amplification of proteins, unlike nucleic acids, is not possible, hindering single-molecule sequencing.
  • Existing methods face challenges in sensitivity and scalability for complex proteomic samples.

Purpose of the Study:

  • To develop a proof-of-concept method for amplifiable protein fingerprinting.
  • To overcome the limitation of direct protein amplification.
  • To enable ultrasensitive protein identification from complex samples.

Main Methods:

  • Residue-resolved DNA barcoding of peptides and proteins.
  • Composition code counting via quantitative PCR amplification of DNA barcodes.
  • Development of a database searching algorithm for protein identification.
  • Design of residue-specific DNA barcoding and amplification workflows.

Main Results:

  • Demonstrated theoretical feasibility of AmproCode via computational simulations of proteome and secretome coverage.
  • Successfully identified synthetic model peptides at the fmol/L level using the developed workflow.
  • Established the foundation for an unprecedented amplifiable protein fingerprinting method.

Conclusions:

  • AmproCode offers a novel approach to protein identification by creating amplifiable fingerprints.
  • The method enables ultrasensitive detection of proteins, even at trace levels.
  • AmproCode holds potential for single-molecule identification of proteins in complex samples and advancing protein sequencing technologies.