Related Experiment Video

Updated: Aug 9, 2026

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary
14:53

Capillary Electrophoresis Separation of Monoclonal Antibody Isoforms Using a Neutral Capillary

Published on: January 16, 2017

A rapid method for comparing monoclonal antibodies by limited proteolysis and electrophoresis

D Weissman, T L Rothstein, A Marshak-Rothstein

    Hybridoma
    |January 1, 1985
    PubMed

    More Related Videos

    Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope08:09

    Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

    Published on: March 24, 2017

    Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method07:58

    Highly Sensitive and Quantitative Detection of Proteins and Their Isoforms by Capillary Isoelectric Focusing Method

    Published on: September 19, 2018

    Related Concept Videos

    Western Blotting01:15

    Western Blotting

    Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
    The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
    Enzyme-Linked Immunosorbent Assay01:33

    Enzyme-Linked Immunosorbent Assay

    In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
    There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.