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Inflammation01:38

Inflammation

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Overview
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Obesity01:24

Obesity

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The Body Mass Index (BMI) is a numerical value derived from a person's weight and height, used to categorize individuals into weight ranges. It is calculated using the formula: weight in kilograms divided by height in meters squared. Obesity is a health condition characterized by excessive accumulation of adipose tissue that poses health risks, often diagnosed with a BMI ≥ 30. This excess fat storage occurs when surplus dietary calories are converted into triglycerides and stored in...
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Related Experiment Video

Updated: Jun 19, 2025

Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
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Lysozyme 1 Inflamed CCR2+ Macrophages Promote Obesity-Induced Cardiac Dysfunction.

Lai Zhang1,2,3, Huian Han1,2, Andi Xu4

  • 1Department of Pathophysiology (L.Z., H.H., S.F., J.Z., W.C., Y.Y., J.L., H.B., J.B., X.Z., X.L., Q.Y., H.Z., Q.C.), Nanjing Medical University, Jiangsu, China.

Circulation Research
|July 26, 2024
PubMed
Summary
This summary is machine-generated.

Obesity triggers cardiac inflammation via CCR2+ macrophages transitioning to a pro-inflammatory state. Lysozyme 1 activates NF-κB signaling, driving heart dysfunction, and may be a therapeutic target.

Keywords:
cardiomyopathiescardiovascular diseasesinflammationmacrophagesobesity

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Area of Science:

  • Immunology
  • Cardiovascular Biology
  • Metabolic Diseases

Background:

  • Macrophages play a critical role in obesity-associated cardiovascular diseases, characterized by inflammation.
  • The precise mechanisms of macrophage involvement in obesity-induced cardiac inflammation remain unclear.
  • This study investigates key macrophage populations and molecular pathways in obesity-related cardiac dysfunction.

Purpose of the Study:

  • To identify the specific macrophage population driving obesity-induced cardiac dysfunction.
  • To elucidate the molecular mechanisms underlying macrophage-mediated cardiac inflammation in obesity.
  • To explore the role of lysozyme 1 in obesity-induced cardiac inflammation.

Main Methods:

  • Single-cell RNA-sequencing of cardiac macrophages to analyze heterogeneity.
  • Genetic ablation of C-C chemokine receptor 2 (CCR2)+ macrophages to assess functional impact.
  • Molecular assays including ChIP-PCR and luciferase assays to define signaling pathways.

Main Results:

  • Cardiac macrophages, specifically CCR2+ cells, shift from homeostasis to a pro-inflammatory phenotype in obesity.
  • Ablation of CCR2+ macrophages or CCR2 itself ameliorates obesity-induced cardiac dysfunction.
  • The CCR2/activating transcription factor 3/lysozyme 1/NF-κB signaling axis mediates this inflammatory shift, with lysozyme 1 activating NF-κB.

Conclusions:

  • Lysozyme 1 acts as a transcription activator for NF-κB, promoting inflammation and cardiac dysfunction in obesity.
  • Targeting lysozyme 1 could offer a diagnostic and therapeutic strategy for obesity-induced heart disease.