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Related Experiment Videos

Sodium hypochlorite as a tissue solubilizing agent compatible with liquid scintillation counting.

R F Puchalski, D K Jasper

    The International Journal of Applied Radiation and Isotopes
    |July 1, 1985
    PubMed
    Summary

    Hypochlorite effectively prepares radiolabeled biological samples for liquid scintillation counting by solubilizing tissues. Acidified stannous chloride addition minimizes chemiluminescence, enabling accurate quantitation of incorporated molecules.

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    Area of Science:

    • Biochemistry
    • Analytical Chemistry
    • Radiochemistry

    Background:

    • Liquid scintillation counting is a common method for quantifying radiolabeled molecules in biological samples.
    • Sample preparation is crucial for efficient and accurate counting.
    • Traditional methods may have limitations in solubilizing and decolorizing diverse biological matrices.

    Purpose of the Study:

    • To investigate the efficacy of hypochlorite as a reagent for preparing biological samples for liquid scintillation counting.
    • To assess hypochlorite's ability to solubilize and decolorize various biological tissues.
    • To address and mitigate chemiluminescence induced by hypochlorite treatment.

    Main Methods:

    • Treatment of diverse biological samples (animal tissues, plant material) with hypochlorite.

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  • Evaluation of solubilization and decolorization efficiency.
  • Assessment of hypochlorite-induced chemiluminescence.
  • Addition of acidified stannous chloride to counting cocktails to suppress chemiluminescence.

    • Main Results:

    • Hypochlorite effectively solubilized and decolorized a range of animal tissues.
    • Fat aggregates, cartilaginous tissue, and plant material showed resistance to hypochlorite digestion.
    • Hypochlorite induced chemiluminescence, which was successfully alleviated by adding acidified stannous chloride.
    • The developed method allows for quantitation of biologically incorporated radiolabeled molecules.

    Conclusions:

    • Hypochlorite is a viable reagent for preparing many biological samples for liquid scintillation counting.
    • The addition of acidified stannous chloride effectively controls hypochlorite-induced chemiluminescence.
    • This modified procedure offers a versatile approach for quantifying radiolabeled molecules in various experimental contexts.