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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

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Related Experiment Video

Updated: Jun 19, 2025

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery

Published on: January 15, 2018

Copolymer-Coated Gold Nanoparticles: Enhanced Stability and Customizable Functionalization for Biological Assays.

Dario Brambilla¹, Federica Panico¹, Lorenzo Zarini¹

¹Institute of Chemical and Technological Science "Giulio Natta", National Research Council of Italy, Via Privata Mario Bianco 9, 20131 Milan, Italy.

|July 26, 2024

Summary

This summary is machine-generated.

This study developed a novel coating for gold nanoparticles (AuNPs) using N,N-dimethylacrylamide-based copolymers. This enhances AuNP stability and allows for easy biomolecule functionalization for improved biosensing applications.

Keywords:

DNA bioassay biomolecule functionalization coating gold nanoparticles microarray polymer streptavidin

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Last Updated: Jun 19, 2025

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Area of Science:

Nanotechnology
Biotechnology
Materials Science

Background:

Gold nanoparticles (AuNPs) are crucial in diagnostics and medicine due to unique optical properties.
Functionalized AuNPs can suffer from instability and non-specific binding in biological settings.
Improving AuNP stability and specificity is key for reliable biosensing.

Purpose of the Study:

To enhance the stability and functionalization of gold nanoparticles (AuNPs) using novel copolymer coatings.
To enable precise biomolecule conjugation for advanced biosensing applications.
To develop a versatile functionalization scheme for AuNPs.

Main Methods:

Coating citrate-stabilized and silica-coated AuNPs with N,N-dimethylacrylamide-based copolymers.
Assessing AuNP stability using spectrophotometry, nanoparticle tracking analysis, and transmission electron microscopy.
Utilizing click chemistry with azide-containing polymers for biomolecule attachment.

Main Results:

Identified two copolymers that provide stable AuNP coatings.
Demonstrated successful functionalization of AuNPs with ssDNA and streptavidin.
Verified the enhanced stability and functionality of modified AuNPs in biological assays.

Conclusions:

A robust and flexible functionalization scheme for AuNPs was developed.
The N,N-dimethylacrylamide-based copolymer coating enhances AuNP stability and allows for straightforward modification.
The developed method offers a versatile platform for various biosensing applications.