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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

386
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Photoluminescence: Fluorescence and Phosphorescence01:23

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Photoluminescence is a process where a molecule absorbs light energy and re-emits it in the form of light. This phenomenon occurs when a substance absorbs photons, promoting its electrons to higher energy level excited states, followed by a relaxation process in which the electrons return to their original ground state energy levels and emit light. Photoluminescence is widely observed in various materials, including semiconductors, and organic and inorganic compounds.
A pair of electrons in a...
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Luminescence Probes in Bio-Applications: From Principle to Practice.

Tao Yan1, Fan Weng1, Yang Ming1

  • 1Department of Cardiovascular Surgery, Zhongshan Hospital Fudan University, Shanghai 200032, China.

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|July 26, 2024
PubMed
Summary
This summary is machine-generated.

This review explores luminescence probes for bioanalysis, detailing their working principles and applications in detecting biomolecules for biological research and medical diagnostics. It covers fluorescence, bioluminescence, chemiluminescence, and other advanced probes.

Keywords:
bio-applicationfluorescenceluminescence probephotoacoustic

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Area of Science:

  • Biomedical Optics
  • Analytical Chemistry
  • Molecular Imaging

Background:

  • Optical imaging-based bioanalysis has advanced significantly.
  • Luminescence probes are crucial for detecting and tracing biomolecules in biological systems.
  • Understanding biomolecule roles requires advanced detection methods.

Purpose of the Study:

  • To review the working principles of various luminescence probes.
  • To summarize recent advances in bio-applications of luminescence probes.
  • To provide a comprehensive understanding of modern luminescence probe technologies.

Main Methods:

  • Review of current literature on luminescence probes.
  • Categorization of luminescence probes based on emission type.
  • Summarization of bio-applications by detection and therapeutic use.

Main Results:

  • Detailed description of fluorescence, bioluminescence, chemiluminescence, afterglow, photoacoustic, and Cerenkov luminescence probes.
  • Overview of applications including metal ion detection, secretion monitoring, in vivo imaging, and therapeutic interventions.
  • Highlighting the versatility and progress in luminescence probe development.

Conclusions:

  • Luminescence probes are essential tools in modern bioanalysis and molecular imaging.
  • Continued development promises enhanced sensitivity and specificity for biological studies.
  • These probes offer significant potential for diagnostics and targeted therapies.