Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Decision Making: Traditional Method

Decision Making: Traditional Method

The process of hypothesis testing based on the traditional method includes calculating the critical value, testing the value of the test statistic using the sample data, and interpreting these values.
First, a specific claim about the population parameter is decided based on the research question and is stated in a simple form. Further, an opposing statement to this claim is also stated. These statements can act as null and alternative hypotheses, out of which a null hypothesis would be a...

Interfacial Electrochemical Methods: Overview

Interfacial Electrochemical Methods: Overview

Interfacial electrochemical methods focus on the phenomena occurring at the boundary between an electrode and a solution, as opposed to bulk methods that concentrate on the solution's overall properties. These interfacial methods are classified as either static or dynamic based on the presence of a nonzero current in the electrochemical cell and the consistency of analyte concentrations. Static methods, such as potentiometry, measure the cell's potential without any significant current passing...

Mechanistic Models: Compartment Models in Algorithms for Numerical Problem Solving

Mechanistic Models: Compartment Models in Algorithms for Numerical Problem Solving

Mechanistic models play a crucial role in algorithms for numerical problem-solving, particularly in nonlinear mixed effects modeling (NMEM). These models aim to minimize specific objective functions by evaluating various parameter estimates, leading to the development of systematic algorithms. In some cases, linearization techniques approximate the model using linear equations.
In individual population analyses, different algorithms are employed, such as Cauchy's method, which uses a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Uncovering MYOF as a novel therapeutic target in glioblastoma: mechanistic insights and drug discovery.

Cell death discovery·2026

Same author

Therapeutic targeting of the conserved region within the low-complexity domain of TDP-43 is neuroprotective and extends survival in amyotrophic lateral sclerosis mice.

Nature aging·2026

Same author

Serum ferritin as a potential biomarker for distinguishing advanced-stage colorectal cancer: a retrospective cohort study.

PeerJ·2026

Same author

Study of the dual mechanism of chromium migration in various biochar-treated soils during electrokinetic remediation.

Environmental technology·2026

Same author

A Novel 3D-Printed Strut-Type Prosthesis Reconstructs the Hemicortical Defect After Distal Femur Parosteal Osteosarcoma Resection: The Mid-Term Follow-Up Outcome.

Orthopaedic surgery·2026

Same author

Association of systemic inflammation response index with postoperative clinical outcomes among operated patients with aortic dissection: a systematic review with meta-analysis.

Journal of thoracic disease·2026

Search research articles

Related Experiment Video

Updated: Jun 9, 2026

Primer-Free Aptamer Selection Using A Random DNA Library

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Aptamer Screening: Current Methods and Future Trend towards Non-SELEX Approach.

Zhihui Fang¹, Xiaorui Feng¹, Fan Tang¹

¹Key Laboratory of Specialty Agri-Products Quality and Hazard Controlling Technology of Zhejiang Province, College of Life Sciences, China Jiliang University, Hangzhou 310018, China.

|July 26, 2024

Summary

This review explores novel aptamer selection methods beyond traditional SELEX. Non-SELEX approaches simplify procedures and reduce costs for DNA, RNA, and XNA aptamer development.

Keywords:

RNA aptamer XNA aptamer aptamer screening high-resolution partitioning non-SELEX methods

More Related Videos

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Related Experiment Videos

Last Updated: Jun 9, 2026

Primer-Free Aptamer Selection Using A Random DNA Library

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Area of Science:

Biotechnology and Molecular Biology
Nucleic Acid Chemistry

Background:

Aptamers are crucial nucleic acid-based molecules for biosensing, drug development, and diagnostics.
Traditional aptamer selection relies on Systematic Evolution of Ligands by Exponential Enrichment (SELEX).
RNA and XNA aptamer selection face challenges including complex enzymatic processes and low amplification efficiency.

Purpose of the Study:

To review recent advances in aptamer selection methodologies.
To provide an outlook on future developments in non-SELEX aptamer selection.
To highlight methods simplifying aptamer screening and reducing experimental costs.

Main Methods:

Overview of traditional SELEX methods for DNA aptamer screening.
Discussion of current screening methods and challenges for RNA and XNA aptamers.
Categorization and analysis of non-SELEX methods into immobilized and non-immobilized formats.

Main Results:

SELEX is effective but complex for RNA and XNA aptamers.
Non-SELEX methods offer simplified and cost-effective alternatives.
High-resolution partitioning is key for improving non-SELEX efficiency and accuracy.

Conclusions:

The future of aptamer selection trends towards non-SELEX approaches.
Non-SELEX methods eliminate the need for nucleic acid amplification.
Further development in partitioning techniques will enhance non-SELEX aptamer discovery.