Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oogenesis02:07

Oogenesis

63.6K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Editorial Expression of Concern: Repeated ovarian stimulation does not affect the expression level of proteins involved in cell cycle control in mouse ovaries and fallopian tubes.

Journal of assisted reproduction and genetics·2026
Same author

Ovarian Reproductive and Endocrine Function Investigated in Microgravity Environment.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Transvaginal versus transabdominal extraction techniques in laparoscopic surgery: a systematic review and meta-analysis.

Minimally invasive therapy & allied technologies : MITAT : official journal of the Society for Minimally Invasive Therapy·2026
Same author

Hormonal therapies for menstrual management in adolescents with medical complexity.

Evidence-based nursing·2026
Same author

Comparative genomics of Idotea balthica populations: Evolutionary divergence between Mediterranean and Baltic lineages.

Marine genomics·2026
Same author

Communicating Down syndrome: professional insensitivity must not bring down the parental experience.

Evidence-based nursing·2026

Related Experiment Video

Updated: Jun 19, 2025

Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model
09:44

Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model

Published on: March 5, 2022

2.9K

Inositol Restores Appropriate Steroidogenesis in PCOS Ovaries Both In Vitro and In Vivo Experimental Mouse Models.

Valeria Fedeli1,2,3, Vittorio Unfer3,4, Simona Dinicola2,3

  • 1Department of Experimental Medicine, University La Sapienza, 00185 Rome, Italy.

Cells
|July 26, 2024
PubMed
Summary
This summary is machine-generated.

Myo-inositol and D-chiro-inositol, at a 40:1 ratio, effectively treat polycystic ovary syndrome (PCOS) by restoring hormone balance. This treatment up-regulates aromatase and FSH receptor levels while reducing excess androgens in PCOS models.

Keywords:
D-chiro-InositolFSH receptoraromatasemyo-Inositolpolycystic ovary syndrome (PCOS)steroidogenesis

More Related Videos

Author Spotlight: Investigating the Mechanisms and Inducing Models of Polycystic Ovary Syndrome
04:49

Author Spotlight: Investigating the Mechanisms and Inducing Models of Polycystic Ovary Syndrome

Published on: July 5, 2024

979
A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome
08:20

A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome

Published on: October 2, 2018

11.2K

Related Experiment Videos

Last Updated: Jun 19, 2025

Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model
09:44

Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model

Published on: March 5, 2022

2.9K
Author Spotlight: Investigating the Mechanisms and Inducing Models of Polycystic Ovary Syndrome
04:49

Author Spotlight: Investigating the Mechanisms and Inducing Models of Polycystic Ovary Syndrome

Published on: July 5, 2024

979
A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome
08:20

A Hyperandrogenic Mouse Model to Study Polycystic Ovary Syndrome

Published on: October 2, 2018

11.2K

Area of Science:

  • Endocrinology
  • Reproductive Biology
  • Metabolic Disorders

Background:

  • Androgen excess is a hallmark of polycystic ovary syndrome (PCOS).
  • FSH receptor (FSHR) and aromatase (CYP19A1) activity are crucial in PCOS pathogenesis.
  • Myo-inositol (myo-Ins) and D-chiro-inositol (D-chiro-Ins) show potential in reactivating CYP19A1 activity.

Purpose of the Study:

  • To investigate the effects of myo-Ins and D-chiro-Ins on the steroidogenic pathway in a murine PCOS model.
  • To analyze the impact of inositol treatment on the expression of Fshr, androgenic, and estrogenic enzymes.
  • To evaluate the efficacy of a 40:1 myo-Ins to D-chiro-Ins ratio in rescuing PCOS features.

Main Methods:

  • A PCOS model was induced in CD1 mice using a continuous light regimen for 10 weeks.
  • Isolated Theca (TCs) and Granulosa cells (GCs), along with ovaries, were analyzed.
  • Real-time PCR was used to assess the expression of Fshr, Cyp19a1, and androgenic/estrogenic enzymes following inositol treatment.

Main Results:

  • Myo-Ins and D-chiro-Ins (40:1 ratio) positively modulated TCs' steroidogenic activity and GCs' expression of Cyp19a1 and Fshr.
  • In vivo administration of inositols (40:1 ratio) significantly increased Cyp19a1 and Fshr expression.
  • Gene expression changes correlated with altered serum hormone levels, indicating successful PCOS feature rescue.

Conclusions:

  • Myo-inositol and D-chiro-inositol, particularly in a 40:1 ratio, are effective in managing PCOS.
  • The 40:1 inositol formula rescues PCOS phenotypes by enhancing aromatase and FSHR levels.
  • This treatment strategy successfully down-regulates androgen excess originating from TCs in PCOS.