Search research articles

Related Concept Videos

DNA as a Genetic Template

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

Position-effect Variegation

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.

Gene Conversion

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...

DNA-only Transposons

DNA-only Transposons

DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

DNA as a Genetic Template

DNA as a Genetic Template

Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Plasma membrane accessible cholesterol is regulated by ACC1 and lipid droplets.

bioRxiv : the preprint server for biology·2025

Same author

Integrating FRET and Molecular Dynamics Simulation for Single-Molecule Aptameric Detection of Staphylococcus aureus IsdA Surface Protein.

Biotechnology journal·2025

Same author

Multiplexed Single-Molecule Detection of Nucleic Acid Biomarkers with a Distance-Tuned Single FRET Pair.

Analytical chemistry·2025

Same author

Fluorescence Analysis of Quinine in Commercial Tonic Waters.

Methods and protocols·2025

Same author

Characterization of a novel oronasal-restricted nicotine vaping self-administration model in mice.

Neuropharmacology·2025

Same author

Single-Molecule FRET-Based Multiplexed Detection.

Methods in molecular biology (Clifton, N.J.)·2024

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: May 20, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Fluorescence-Based Multimodal DNA Logic Gates.

Chamika Harshani Algama¹, Jamil Basir¹, Kalani M Wijesinghe¹

¹Department of Chemistry, Virginia Commonwealth University, Richmond, VA 23284, USA.

Nanomaterials (Basel, Switzerland)

|July 26, 2024

Summary

Researchers developed simple DNA logic gates for molecular computing. These DNA logic gates utilize a proximity quenching and strand displacement strategy, enabling multimodal operations for advanced biosensing and biological computers.

Keywords:

DNA logic gates fluorophore four-way DNA multimodal quencher

More Related Videos

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

Related Experiment Videos

Last Updated: May 20, 2026

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Plasmid-derived DNA Strand Displacement Gates for Implementing Chemical Reaction Networks

Published on: November 25, 2015

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

Area of Science:

Molecular Biology
Nanotechnology
Biocomputing

Background:

DNA structures offer potential for molecular devices and computation systems.
Current DNA logic gate implementations are limited due to complex designs and working principles.

Purpose of the Study:

To design simple, multimodal DNA logic gates for versatile operations.
To establish a foundation for advanced molecular devices and computation systems.

Main Methods:

Designed simple four-way DNA logic gates.
Utilized proximity quenching of fluorophore-quencher pairs.
Employed toehold-mediated strand displacement (TMSD) strategy for gate operation.

Main Results:

Demonstrated fluorescence output solely dependent on oligonucleotide input, indicating successful gate opening.
Successfully created multimodal logic gates (YES, AND, OR, and combinations) with tunable displacement initiation sites.
Validated the effectiveness of the TMSD strategy in conjunction with proximity quenching.

Conclusions:

The developed four-way DNA logic gates provide a simple yet powerful platform for molecular operations.
This strategy holds significant promise for the development of biological computers.
The gates are suitable for next-generation smart molecular circuits with integrated biosensing capabilities.